Abstract
Penicillium citrinum Thom, isolated from the sclerotia of Sderotinia minor, was cultured in a broth of Czapek-Dox for 4 to 8 weeks. The filtrate obtained was incorporated into potato dextrose agar or Czapek-Dox agar at different concentrations (v/v). The amended media were tested for mycelial growth of S. minor and other pathogens. Mycelial growth of S. minor was completely inhibited on media amended with 20% (v/v) filtrate of P. citrinum, and considerable inhibition of S. minor occurred at 10 and 15% concentrations. Mycelial growth of S. major, Sclerotium rolfsii, Rhizoctonia solani (AG-4) was inhibited by similar concentrations of filtrate of P. citrinum. Inhibitor(s) in the filtrate were extracted with ethyl acetate and tentatively identified as citrinin. Citrinin was shown to be an active component in the filtrate against mycelial growth of S. minor, S. major and Sclerotium rolfsii.
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References
Hald B, Krogh P. Analysis and chemical confirmation of citrinin in Barley. Journal of Assoc of Official Analytical Chemists 1973; 56:1440–1443.
Bilaj VI. Mikroskopicheskie griby-produtsenty antibiootikov (microscopic fungi-producers of antibiotics) in Izdat. Akaemii nauk ukrainskoj. SSR, Kiev 1961; 24.
Caldwell RW, Tuite J, Carlton WW. Pathogenicity of Penicilia to corn ears. Phytopathology 1981; 71:175–180.
Ciegler A, Kurtzman CP. Penicillic acid production by blue-eye fungi on various agricultural commodities. Applied Microbiol 1970; 20:761–764.
Hetherington AC, Raistrick H. Citrinin; an antibiotic produced by Penicillium citrinum. Phil Trans Roy Soc (London) B 1931; 220:269–296.
Johann H, Holbert JK, Dickson JG. Further studies on Penicillium injury to corn. J Agric Res 1931; 43:757–790.
Melouk HA, Chanakira FA, Conway KE. Inhibition of Sclerotinia minor by Penicillium citrinum. (Abstract). Phytopathology 1985; 75:502.
Raistrick H, Smith G. Studies in the Biochemistry of Microorganisms. The metabolic products of Aspergillus terreus Thom. A new mould metabolic product-Terrein. Biochemical Journal 1935; 29:606–611.
Rodig OR, Ellis LC, Glover IT. The Biosynthesis of citrinin in Penicillium citrinum I and II. Production and Degradation of citrinin. Biochemistry 1966; 5:2451–2462.
Scott PM, Van Walbeek W, Harwig J, Fennell DI. Occurrence of a mycotoxin, Ochratoxin A in Wheat and Isolation of Ochratoxin A and citrinin producing strains of Penicillium viridicatum. Can J Plant Science 1970; 50:583–585.
Scott PM, Van Walbeek W, Kennedy B, Anyeti D. Mycotoxins and toxigenic fungi in grains and other agricultural products. J Agric Food Chemistry 1972; 20:1103–1109.
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Cooperative investigation of U.S. Department of Agriculture, Agriculture Research Service and Oklahoma State University. Journal Article No. 4989, Oklahoma Agricultural Experiment Station, Oklahoma State University, Stillwater.
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Melouk, H.A., Akem, C.N. Inhibition of growth of Sclerotinia minor and other pathogens by citrinin in the filtrate of Penicillium citrinum . Mycopathologia 100, 91–96 (1987). https://doi.org/10.1007/BF00467100
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DOI: https://doi.org/10.1007/BF00467100