Summary
Using high performance liquid chromatography, the presence of N6-methyladenine has been found at a level of 0.1 mol percent in DNA extracted from Penicillium chysogenum. No 5-methylcytosine was detected. A mutant strain HP547, which is sensitive to the lethal effects of N-methyl-N1-nitro-N-nitrosoguanidine, methylmethane sulphonate and the base analogue 2-aminopurine shows an increased spontaneous mutation rate and no detectable DNA methylation. Comparison of restriction enzyme digests of wild type and undermethylated strains indicated that methylation was occurring at a different sequence to that of the Dam methylase system of E. coli.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Adams RLP, Burdon RH (1982) CRC Crit Rev Biochem 13:349 to 384
Ditchburn P, Giddings B, Macdonald KD (1974) J Appl Bacteriol 37:515–523
Ehrlich M, Wang RYH (1981) Science 212:1350–1357
Glickman B, Radman M (1980) Proc Natl Acad Sci USA 77:1063 to 1066
Glickman B, van den Elsan P, Radman M (1978) Mol Gen Genet 163:307–312
Hattman S, Gold E, Plotnik A (1972) Proc Natl Acad Sci USA 69:187–190
Hattman S, Kenny C, Berger L, Pratt K (1978) J Bacteriol 135:1156–1157
Holt G, Adlard M, Lisboa E, Rogers ME, Rogers SD, Saunders G, Smith T (1984) Biochem Soc Trans 12:577–580
Lacks S, Greenberg B (1977) J Mol Biol 114:153–168
Marinus MG, Morris NR (1973) J Bacteriol 114:1143–1150
Raper KB, Alexander DF (1945) J Elisha Mitchell Sci Soc 61:74–113
Rogers SD, Holt G (1984) Appl Microbiol Biotechnol 20:251–255
Saunders G, Rogers ME, Adlard MW, Holt G (1984) Mol Gen Genet 1984:343–345
Smith JD, Arber W, Kuhnlein U (1972) J Mol Biol 63:1–8
Tamane M, Antegueia F, Villaneuva JR, Santos T (1983) J Gen Microbiol 129:2079–2089
Wagner I, Capesius I (1981) Biochim Biophys Acta 654:52–56
Whittacker PA, Hardman N (1980) Biochem J 191:859–862
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rogers, S.D., Rogers, M.E., Saunders, G. et al. Isolation of mutants sensitive to 2-amiriopurine and alkylating agents and evidence for the role of DNA methylation in Penicillium chrysogenum . Curr Genet 10, 557–560 (1986). https://doi.org/10.1007/BF00447390
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00447390