Abstract
Chromatium vinosum cells form a vesicular type intracytoplasmic membrane system during phototrophic growth on thiosulfate.—An enzyme protein transferring electrons from thiosulfate to cytochromes of type c was enriched from S-144. The colorless thiosulfate: cytochrome c oxidoreductase was characterized by a molecular weight of 36,000 (after dodecylsulfate treatment) and 35,000 (by gel filtration). Isoelectric focusing revealed a pI range of 4.4 to 4.7. Apparent K m values for the cytochromes tested were in the μM range. — The endogenous electron acceptor compound, isolated from the chromatophore fraction P-144, was found to be a membrane-bound cytochrome c-552. The homogeneous cytochrome protein had an average pI value of 4.65 and a molecular weight of 71,500 determined by gel filtration. By dodecylsulfate electrophoresis it was cleaved into two proteins representing particle weights of 45,000 and 20,000.
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Abbreviations
- HiPIP:
-
high potential nonheme iron protein
- IEF:
-
isoelectric focusing
- SDS:
-
dodecylsulfate, sodium salt
- Temed:
-
N,N,N′,N′-tetramethylethylenediamine
References
Bartsch RG, Meyer TE, Robinson AB (1968) Complex c-type cytochromes with bound flavin. In: K Okunuki, MD Kamen, Y Sekuzu (eds) Structure and function of cytochromes. University of Tokyo Press, Tokyo, pp 443–451
Bartsch RG (1971) Cytochromes: Bacterial. In: A San Pietro (ed) Methods of enzymology, XXIII, A. Academic Press, New York, pp 344–363
Bartsch RG (1978) Cytochromes. In: RK Clayton, WR Sistrom (eds) The photosynthetic bacteria. Plenum Press, New York, pp 249–279
Bose SK (1963) Media for anaerobic growth of photosynthetic bacteria. In: H Gest, A San Pietro, LP Vernon (eds) Bacterial photosynthesis. The Antioch Press, Yellow Springs, pp 501–510
Fukumori Y, Yamanaka T (1979a) Flavocytochrome c of Chromatium vinosum. J Biochem 85:1405–1414
Fukumori Y, Yamanaka T (1979b) A high-potential nonheme iron protein (HiPIP)-linked, thiosulfate-oxidizing enzyme derived from Chromatium vinosum. Curr Microbiol 3:117–120
Grondelle R van, Duysens LNM, Wel JA van der, Wal HN van der (1977) Function and properties of a soluble c-type cytochrome c-551 in secondary photosynthetic electron transport in whole cells of Chromatium vinosum as studied with flash spectroscopy. Biochim Biophys Acta 461:188–201
Kennel SJ, Kamen MD (1971a) Iron-containing proteins in Chromatium. I. Solubilization of membrane-bound cytochrome. Biochim Biophys Acta 234:458–467
Kennel SJ, Kamen MD (1971b) Iron-containing proteins in Chromatium. II. Purification and properties of cholate-solubilized cytochrome complex. Biochim Biophys Acta 253:153–166
Knobloch K, Gemeinhardt F (1979) On the phospholipids and fatty acids in cell-free fractions from the Rhodospirillaceae Rhodopseudomonas palustris and Rps. spheroides. In: LA Appelquist, C Liljenberg (eds) Advances in the biochemistry and physiology of plant lipids. Elsevier/North-Holland Biomedical Press, Amsterdam
Kusai K, Yamanaka T (1973a) The oxidation mechanisms of thiosulphate and sulfide in Chlorobium thiosulphatophilum: Roles of cytochrome c-551 and cytochrome c-553. Biochim Biophys Acta 325:304–314
Kusai K, Yamanaka T (1973b) A novel function of cytochrome c (555, Chlorobium thiosulfatophilum) in oxidation of thiosulfate. Biochem Biophys Res Commun 51:107–112
Lyric RM, Suzuki I (1970) Enzymes involved in the metabolism of thiosulfate by Thiobacillus thioparus. III. Properties of thiosulfate-oxidizing enzyme and proposed pathway of thiosulfate oxidation. Can J Biochem 48:355–363
Schleifer G, Schmitt W, Knobloch K (1981) The enzymatic system thiosulfate: cytochrome c oxidoreductase from photolithoautotrophically grown Rhodopseudomonas palustris. Arch Microbiol 130:328–333
Schmitt W, Schleifer G, Knobloch K (1980) On the enzymatic system thiosulfate-cytochrome c-oxidoreductase from Chromatium vinosum. Isolation and purification of the acceptor cytochrome c. Hoppe-Seyler's Z Physiol Chem 361:1338
Smith AJ, Lascelles J (1966) Thiosulphate metabolism and rhodanese in Chromatium sp. strain D. J Gen Microbiol 42:357–370
Smith AJ (1966) The role of tetrathionate in the oxidation of thiosulphate by Chromatium sp. strain D. J Gen Microbiol 42:371–380
Sybesma C (1970) Photosynthetic bacteria. In: P Halldal (ed) Photobiology of microorganisms. Wiley-Interscience, London, pp 57–93
Weber K, Osborn M (1969) The reliability of molecular weight determination by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem 244:4406–4412
Yamanaka T, Fukumori Y, Okunuki K (1979) Production of subunits of flavocytochromes c derived from Chlorobium limicola f. thiosulphatofilum and Chromatium vinosum. Anal Biochem 95:209–213
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Schmitt, W., Schleifer, G. & Knobloch, K. The enzymatic system thiosulfate: Cytochrome c oxidoreductase from photolithoautotrophically grown Chromatium vinosum . Arch. Microbiol. 130, 334–338 (1981). https://doi.org/10.1007/BF00425951
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DOI: https://doi.org/10.1007/BF00425951