Abstract
It has been demonstrated that enzymes from Clostridium thermoaceticum catalyze the following reaction in which Fd is ferredoxin and CH3THF is methyltetrahydrofolate.
The system involves hydrogenase, CO dehydrogenase, a methyltransferase, a corrinoid enzyme and other unknown components. Hydrogenase catalyzes the reduction of ferredoxin by H2; CO dehydrogenase then uses the reduced ferredoxin to reduce CO2 to a one-carbon intermediate that combined with CoASH and with a methyl group originating from CH3THF to form acetyl-CoA. It is proposed that these reactions are part of the mechanism which enables certain acetogenic autotrophic bacteria to grow on CO2 and H2.
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References
Andreesen JR, Ljungdahl LG (1974) Nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase from Clostridium thermoaceticum. Purification and properties. J Bacteriol 120:6–14
Blair JA, Saunders JK (1970) A convenient method for the preparation of dl-5-methyltetrahydrofolic and (dl-5-methyl-5,6,7,8-tetrahydropteroyl-L-monoglutamic acid). Anal Biochem 34:376–381
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Chen IS, Blanchard DK (1979) A simple hydrogenase-linked assay for ferredoxin and flavodioxin. Anal Biochem 93:216–222
Diekert G, Ritter M (1983) Purification of the nickel-protein carbon monoxide dehydrogenase of Clostridium thermoaceticum. FEBS Lett 151:41–44
Drake HL (1980) Demonstration of hydrogenase in extracts of the homoacetate-fermenting bacterium Clostridium thermoaceticum. J Bacteriol 150:702–709
Drake HL, Hu SI, Wood HG (1980) Purification of carbon monoxide dehydrogenase, a nickel enzyme from Clostridium thermoaceticum. J Biol Chem 255:7174–7180
Drake HL, Hu SI, Wood HG (1981) Purification of five components from Clostridium thermoaceticum which catalyzes synthesis of acetate from pyruvate and methyltetrahydrofolate. Properties of phosphotransacetylase. J Biol Chem 256:11137–11144
Eden G, Fuchs G (1982) Total synthesis of acetyl coenzyme A involved in autotrophic CO2 fixation. Arch Microbiol 133:66–74
Elliott JI, Ljungdahl LG (1982) Isolation and characterization of an Fe8-S8 ferredoxin (ferredoxin II) from Clostridium thermoaceticum. J Bacteriol 151:328–333
Erbes DL, Burns RH (1978) The kinetics of methyl viologen oxidation and reduction by the hydrogenase from Clostridium thermoaceticum. Biochim Biophys Acta 525:45–54
Hu SI, Drake HL, Wood HG (1982) Synthesis of acetyl coenzyme A from carbon monoxide, methyltetrahydrofolate, and coenzyme A by enzymes from Clostridium thermoaceticum. J Bacteriol 149:440–448
Hu SI, Wood HG (1983) The pathway of autotrophic growth by bacteria using CO: The corrinoid protein. ASBC Meeting, San Francisco, Poster No. 2209
Kerby R, Zeikus JG (1983) Growth of Clostridium thermoaceticum on H2/CO2 or CO as energy source. Curr Microbiol 8:27–30
Kleiner D, Burris RH (1970) The hydrogenase of Clostridium pasteurianum kinetic studies and the role of molybdenum. Biochim Biophys Acta 212:417–427
Ljungdahl LG, Andreesen JR (1975) Tungsten, a component of active formate dehydrogenase from Clostridium thermoaceticum. FEBS Lett 59:8–11
Ljungdahl LG, Wood HG (1982) Acetate synthesis. In: Dolphin D (ed) Vitamin B12. Academic Press, New York, pp 165–202
Meyer O (1982) Chemical and spectral properties of carbon monoxide: Methylene blue oxidoreductase; the molybdenum-containing iron sulfur flavoprotein from Pseudomonas carboxydovorans. J Biol Chem 257:1333–1341
Morrissey JH (1981) Silver strain of proteins in gels: A modified procedure with enhanced uniform sensitivity. Anal Biochem 117:301–307
Parker DJ, Wu TF, Wood HG (1971) Total synthesis of acetate from CO2: Methyltetrahydrofolate, an intermediate, and a procedure for separation of the folates. J Bacteriol 108:770–776
Phares EF (1951) Degradation of labeled propionic and acetic acids. Arch Biochem Biophys 33:173–178
Pines MJ (1958) Methane fermentation of formate by Methanobacillus omelianskii. J Bacteriol 75:356–359
Ragsdale SW, Ljungdahl LG, Der Vartanian DV (1982) EPR evidence for nickel-substrate interaction in carbon monoxide dehydrogenase from Clostridium thermoaceticum. Biochem Biophys Res Comm 108:658–663
Ragsdale SW, Clark JE, Ljungdahl LG, Lundie LL, Drake HL (1983a) Properties of purified carbon monoxide dehydrogenase from Clostridium thermoaceticum, a nickel, iron-sulfur protein. J Biol Chem 258:2364–2369
Ragsdale SW, Ljungdahl LG, Der Vartanian DV (1983b) Isolation of the carbon monoxide dehydrogenase from Acetobacterium woodii and comparison of its properties with the Clostridium thermoaceticum enzyme. J Bacteriol 155:1224–1237
Schulman M, Ghambeer RK, Ljungdahl LG, Wood HG (1973) Total synthesis of acetate from CO2. VII. Evidence with Clostridium thermoaceticum that the carboxyl of acetate is derived from carboxyl of pyruvate by transcarboxylation and not by fixation of CO2. J Biol Chem 248:6255–6261
Thauer RK (1972) CO2-reduction to formate by NADPH. The initial step in the total synthesis of acetate from CO2 in Clostridium thermoaceticum. FEBS Lett 27:111–115
Welty FK, Wood HG (1978) Purification of the “corrinoid” enzyme involved in the synthesis of acetate by Clostridium thermoaceticum. J Biol Chem 253:5832–5838
Wood HG, Drake HL, Hu SI (1982) Studies with Clostridium thermoaceticum and resolution of the pathway used by acetogenic bacteria that grow on carbon monoxide or carbon dioxide and hydrogen. Proc Biochem Symp, Annual Review. Pasadena, CA, pp 28–56
Wrigley NG (1971) Gel electrofocusing. In: Colowick N, Kaplan SP (eds) Methods in enzymology, vol 22. Academic Press, New York, pp 559–569
Zeikus JG (1983) Metabolism of 1-carbon compounds by chemotrophic anaerobes. Adv Microbiol Physiol, vol 24. Academic Press, New York, pp 222–230
Yamamoto J, Saiki T, Liu SM, Ljungdahl LG (1983) Purification and properties of NADP-dependent formate dehydrogenase from Clostridium thermoaceticum, a tungsten-selenium-iron protein. J Biol Chem 258:1826–1832
Yang SS, Ljungdahl LG, LeGall J (1977) A four-iron, four-sulfide ferredoxin with high thermostability from Clostridium thermoaceticum. J Bacteriol 130:1084–1090
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Pezacka, E., Wood, H.G. The synthesis of acetyl-CoA by Clostridium thermoaceticum from carbon dioxide, hydrogen, coenzyme A and methyltetrahydrofolate. Arch. Microbiol. 137, 63–69 (1984). https://doi.org/10.1007/BF00425809
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DOI: https://doi.org/10.1007/BF00425809