Abstract
Phosphate permeability and phosphate metabolism of human erythrocytes have been studied by determining the rates of phosphate influx and efflux, and by measuring concentrations as well as 32P-labelling of intracellular phosphate compounds under different experimental conditions. The following results were obtained:
Temperature coefficients of phosphate efflux and influx are of similar magnitude (4,0–4,5 between 20° and 37° C). Intracellular orthophosphate seems to be most probably the precursor pool of phosphate ions released from the red cells.
The rates of phosphate influx and efflux are considerably reduced by dipyridamole, pyrazolidines, phloretin, reserpine, tannic acid et al., the efflux being in general more strongly influenced than the influx. Since under the same conditions glycolysis and concentrations of intracellular P-compounds remain unaltered, the effects observed can only result from a decrease of phosphate permeability caused by the different substances.
Phosphate influx and efflux are enhanced by EDTA, EGTA, ATP, phytic acid, oxalate et al., but not by omission of Ca++ from the incubation medium. EDTA and EGTA are ineffective, however, in Ca++-free media. In most cases the enhancement of phosphate transfer is probably due to an increase of the membrane permeability only.
Inhibitors of glycolysis (iodoacetate, fluoride, arsenate et al.) do not primarily affect phosphate influx; phosphate efflux, however, becomes remarkably increased due to the progressive elevation of intracellular orthophosphate levels brought about by these inhibitors. Purine nucleosides (adenosine, inosine, guanosine), which likewise have no influence on phosphate influx, considerably reduce the efflux rates. This effect results from the diminution of intracellular orthophosphate in consequence of its intensified esterification.
Elevation of external orthophosphate levels causes a proportional increase of phosphate influx, thus raising the intracellular orthophosphate concentration and by this again augmenting the efflux of phosphate.
From the results obtained it becomes evident that in human erythrocytes uptake and release of phosphate, which are in principle independent of cellular energy metabolism, can be experimentally affected either by alterations of membrane permeability or by changes of internal and external orthophosphate concentrations.
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Herrn Prof. Dr. Dr. h. c. L. Heilmeyer zum 65. Geburtstag gewidmet.
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Wesentliche Ergebnisse der vorliegenden Arbeit werden von Herrn J. Duhm der Medizinischen Fakultät der Universität Freiburg i. Br. als Dissertation vorgelegt.
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Gerlach, E., Deuticke, B. & Duhm, J. Phosphat-Permeabilität und Phosphat-Stoffwechsel menschlicher Erythrocyten und Möglichkeiten ihrer experimentellen Beeinflussung. Pflugers Arch. 280, 243–274 (1964). https://doi.org/10.1007/BF00418367
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DOI: https://doi.org/10.1007/BF00418367