Abstract
Experimental studies of ocular inflammation induced by endotoxin have been performed almost exclusively in rabbits. Although the rat has been considered refractory to both the systemic and local effects of endotoxin, the present study has shown that intravitreal injection of endotoxin evoked a characteristic acute inflammatory response which was reproducible and dose-dependent. In addition, a consensual ocular response in the control eye occurred which was less severe but also dose-dependent. Preliminary investigations support the view that consensual responses of this nature may be mediated by a nervous reflex arc mechanism. Sequential histological analysis revealed a marked polymorphonuclear cell infiltration of most ocular tissues in the early stages, while mononuclear cells predominated after 3 days. Widespread intravascular microthrombi were also observed, particularly in the ciliary vessels. Recovery, however, was usually complete by 7 days.
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Apitz, K.: A study of the generalized Shwartzman phenomenon. J. Immunol. 29, 255–264 (1935)
Ayo, C.: A toxic ocular action. New property of Shwartzman toxins. J. Immunol. 46, 113–132 (1943)
Behrens, M., Manski, W.: Immunogenic uveitis in inbred rats. Ophthalmol. Res. 5, 31–40 (1973)
Beitch, S.R., Eakins, K.E.: Effects of prostaglandins on the intraocular pressure of the rabbit. Br. J. Pharmacol. 37, 158–167 (1969)
Bito, L.Z.: The effects of experimental uveitis on anterior uveal prostaglandin transport and aqueous humor composition. Invest. Ophthalmol. 13, 959–966 (1974)
Bito, L.Z.: Inflammatory effects of endotoxin-like contaminants in commonly used protein preparations. Science 196, 83–85 (1977)
Butler, J.M., Unger, W.G., Hammond, B.R.: Sensory mediation of the ocular response to neutral formaldehyde. Exp. Eye Res. 28, 577–589 (1979)
Butler, J.M.: Substance P as a mediator of the ocular injury response. (Abstr) Association for Eye Research, 20th Meeting, Jouy-en-Josas, France, 1979
Chien, S., Sinclair, D.G., Dellenback, R.J.: Effect of endotoxin on capillary permeability to macromolecules. Am. J. Physiol. 207, 518–522 (1964)
Chando, J.W.: Affinity of endotoxin for membranes. J. Infect. Dis. 128, 5197–5201 (1973)
Guillery, F.: Ueber Ferment-Wirkung des Auges und ihre Beziehungen zu sympathischer Ophthalmie. Arch. Augenheilk. 68, 242–289 (1911)
Horn, R.G.: Evidence for participation of granulocytes in the pathogenesis of the generalised Shwartzmann reaction: A review. J. Infect. Dis. 128, 5134–5142 (1973)
Howes, E.L., McKay, D.G.: Effect of cervical sympathectomy on the ocular response to systemic endotoxin. Proc. Soc. Exp. Biol. Med. 139, 839–844 (1972)
Howes, E.L., McKay, D.G.: Ocular changes in the generalised Shwartzmann reaction. Arch. Ophthalmol. 90, 218–224 (1973)
Howes, E.L., McKay, D.G., Margaretten, W.: The participation of the platelet in the vascular response to endotoxaemia in the rabbit eye. Am. J. Pathol. 70, 25–37 (1973)
Kaley, G., Demopoulos, B.S., Zweifach, H.: Occlusive vascular lesions induced by bacterial endotoxin in kidneys of pregnant rats. Proc. Soc. Exp. Biol. Med. 109, 456–460 (1962)
Kottow, M.H., Seligman, L.J.: Consensual reactions to anterior chamber paracentesis in the rabbit. Am. J. Ophthalmol. 85, 392–399 (1978)
Leplat, G.: Etude de quelques reactions provoquees dans les yeux par une contusion oculaire unilaterale. Ann. Oculist. 161, 87–106 (1924)
Levene, R.Z.: General and ocular effects of endotoxin. Surv. Ophthalmol. 4, 581–592 (1959)
Levene, R.Z., Breinin, C.M.: Ocular effects of endotoxin. Arch. Ophthalmol. 88, 568–577 (1959)
Magitot, A.: Sur le tension oculaire et quelques-unes de ses modifications experimentales. Ann. Oculist. 160, 81–105 (1923)
Morrison, D.C., Cochrane, C.G.: Direct evidence for Hageman factor activation by bacterial lipopolysaccharides. J. Exp. Med. 140, 797–811 (1974)
Nowotny, A.: Molecular aspects of endotoxic reactions. Bact. Revs. 33, 72–98 (1969)
Pappas, G.D., Smelser, G.K.: Studies on the ciliary epithelium and the zonule. Electron microscopic observations on changes induced by alteration of normal aqueous humor formation in the rabbit. Am. J. Ophthalmol. 46, 299–317 (1959)
Perkins, E.S.: Influence of the fifth cranial nerve on the intra-ocular pressure of the rabbit. Br. J. Pharmacol. 37, 158–167 (1969)
Rietschel, E.T.: Chemical structure and biological activity of endotoxins (lipopolysaccharides) and lipid A. Naunyn Schmiedebergs Arch. Pharmacol. 287, 74–84 (1975)
Segawa, K., Smelser, G.K.: Electron microscopy of experimental uveitis. Invest. Ophthalmol. 8, 497–520 (1969)
Taichman, M.S., Uriuhara, T., Movat, H.Z.: Ultrastructural alterations in the local Shwartzman reaction. Lab. Invest. 14, 2160–2176 (1966)
Theiss, W., Hilgard, P., Hayes, H.: Induction of disseminated intravascular coagulation by endotoxin and saline loading in rats. Fibrin deposition and removal. Thromb. Res. 7, 47–58 (1975)
Tilgner, S., Henyrel, E., Meyer, W., Schumann, M.: Ophthalmologische Untersuchungen über die Adjuvanskrankheit der Ratte. Klinische und histologische Befunde. Albrecht v. Graefe's Arch. klin. exp. Ophthalmol. 183, 348–362, 1972, a.
Tilgner, S., Henyrel, E., Meyer, W., Schumann, M.: Ophthalmologische Untersuchungen über die Adjuvanskrankheit der Ratte. Quantitative Daten zum klinischen Bild unter besonderer Berücksichtigung der Residive. Albrecht v. Graefe's Arch. klin. exp. Ophthalmol. 184, 155–166, 1972, b.
Waksman, B.H., Bullinton, S.J.: Studies of arthritis and other lesions induced in rats by injection of mycobacterial adjuvant. Lesions of the eye. Arch. Ophthalmol. 64, 752–762 (1960)
Zygulska-Mach, H., Mach, Z.: Über die Einwirkung von Gammastrahlen auf den Gehalt des Glutathions unter der Ascorbinsäure in Kaninchenlinsen. Klin. Mbl. Augenheil 166, 50–55 (1975)
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This study was supported by National Institute of Health Grants EY-02648-01 and EY-01747 from the National Eye Institute
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Forrester, J.V., Worgul, B.V. & Merriam, G.R. Endotoxin-induced uveitis in the rat. Albrecht von Graefes Arch. Klin. Ophthalmol. 213, 221–233 (1980). https://doi.org/10.1007/BF00417543
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DOI: https://doi.org/10.1007/BF00417543