Abstract
R-Bodies have been found in a recently isolated pseudomonas-like free-living hydrogen oxidizing bacterium. Their isolation, fine structure and chemical composition are described and compared with the R-bodies from the kappa particles (Caedobacter), obligate endosymbionts of Paramecium aurelia. The 2K 1 R-bodies exhibited essential characteristics of the kappa R-bodies; however, their size and some other structural aspects proved that they represent a new type of R-bodies. The presence of phage tail-like particles in cells induced with Mitomycin C is in favour of the hypothesis that the R-bodies might be coded by defective prophages, or by extrachromosomal elements.
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Auling, G., Reh, M., Lee, C.-M., Schlegel, H. G.: Pseudomonas pseudoflava, a new species of hydrogen-oxidizing bacteria: Its differentiation from Pseudomonas flava and other yellow pigmented, gram-negative, hydrogen oxidizing species. Int. J. Syst. Bacteriol. 28, 82–95 (1978).
Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254 (1976)
Dilts, J. A.: Chromosomal and extrachromosal deoxyribonucleic acid from four bacterial endosymbionts derived from stock 51 of Paramecium teiraurelia. J. Bacteriol 129, 885–894 (1977)
Hartree, E. F.: Determination of protein: A modification of the Lowry method that gives a linear photometric response. Anal. Biochem. 48, 422–427 (1972)
Lalucat, J., Mayer, F.: “Spiral-bodies”-intracytoplasmic membraneous structures in a hydrogen oxidizing bacterium. Z. Allg. Mikrobiol. 18, 517–521 (1978)
Lotz, W.: Defective bacteriophages: The phage tail-like particles. In: Progress in molecular and subcellular biology, Vol. 4, (F. E. Hahn, ed.), pp. 53–102. Berlin-Heidelberg-New York: Springer 1976
Pfennig, N.: Rhodopseudomonas globiformis, sp. n. a new species of the Rhodospirillaceae. Arch. Microbiol. 100, 197–206 (1974)
Preer, J. R., Jr., Hufnagel, L. A., Preer, L. B.: Structure and behavior of “R” bodies from killer paramecia. J. Ultrastruct. Res. 15, 131–143 (1966)
Preer, J. R., Jr., Preer, L. B., Jurand, A.: Kappa and other endosymbionts in Paramecium aurelia. Bacteriol. Rev. 38, 113–163 (1974)
Preer, L. B., Jurand, A., Preer, J. R., Jr., Rudman, B. M.: The classes of kappa in Paramecium aurelia. J. Cell Sci. 11, 581–600 (1972)
Quackenbush, R. L.: Phylogenetic relationship of bacterial endosymbionts of Paramecium aurelia: Polynucleotide sequence relationships of 51 kappa and its mutants. J. Bacteriol. 129, 895–900 (1977)
Quackenbush, R. L.: Genetic relationships among bacterial endosymbionts of P. aurelia: Deoxyribonucleotide sequence relationships among members of Caedobacter. J. Gen. Microbiol. 108, 181–187 (1978)
Schlegel, H. G., Kaltwasser, H., Gottschalk, G.: Ein Submersverfahren zur Kultur wasserstoffoxidierender Bakterien: Wachstumsphysiologische Untersuchungen. Arch. Mikrobiol. 38, 209–222 (1961)
Irevelyan, W. E., Harrison, J. S.: Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem. J. 50, 298–303 (1952)
Valentine, R. C., Shapiro, B. M., Stadtman, E. R.: Regulation of glutamine synthease. XII. Electron microscopy of the enzyme from E. coli. Biochemistry 7, 2143–2152 (1968)
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Lalucat, J., Meyer, O., Mayer, F. et al. R-Bodies in newly isolated free-living hydrogen-oxidizing bacteria. Arch. Microbiol. 121, 9–15 (1979). https://doi.org/10.1007/BF00409199
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DOI: https://doi.org/10.1007/BF00409199