Summary
Cell-free extracts of methane-utilizing bacteria (methylobacteria) were examined for the presence of enzymes of the tricarboxylic acid cycle. Representative organisms of the Type II group had a complete set of enzymes for the operation of this cycle. Members of the Type I group lacked α-ketoglutarate dehydrogenase. All the methylobacteria examined had an NADH-oxidase which had the properties of a flavoprotein type enzyme. Glucose-6-phosphate dehydrogenase and gluconate-6-phosphate dehydrogenase was NADP-specific and could only be detected in cell extracts of Type I methylobacteria.
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Davey, J.F., Whittenbury, R. & Wilkinson, J.F. The distribution in the methylobacteria of some key enzymes concerned with intermediary metabolism. Archiv. Mikrobiol. 87, 359–366 (1972). https://doi.org/10.1007/BF00409135
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DOI: https://doi.org/10.1007/BF00409135