Abstract
The synthesis of chitin during germ-tube formation in Candida albicans may be regulated by the first and last steps in the chitin pathway: namely l-glutamine-d-fructose-6-phosphate aminotransferase and chitin synthase. Induction of germ-tube formation with either glucose and glutamine or serum was accompanied by a 4-fold increase in the specific activity of the aminotransferase. Chitin synthase in C. albicans is synthesized as a proenzyme. N-acetyl glucosamine increased the enzymic activity of the activated enzyme 3-fold and the enzyme exhibited positive co-operativity with the substrate, UDP-N-acetylglucosamine. Although chitin synthase was inhibited by polyoxin D (K i =1.2μM) this antibiotic did not affect germination. During germ-tube formation the total chitin synthase activity increased 1.4-fold and the expressed activity (in vivo activated proenzyme) increased 5-fold. These results could account for the reported 5-fold increase in chitin content observed during the yeast to mycelial transformation.
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Abbreviations
- GlcNac:
-
N-acetyl glucosamine
- UDP-GlcNac:
-
UDP-N-acetyl glucosamine
- PMSF:
-
phenylmethylsulphonylfluoride
References
Atkinson, D. E., Hathaway, J. A., Smith, E. C.: Kinetics of regulatory enzymes: Kinetic order of the yeast NAD isocitrate dehydrogenase reaction and a model for the reaction. J. Biol. Chem. 240, 2682–2690 (1965)
Bartnicki-Garcia, S., Lippman, E.: Inhibition of Mucor rouxii by Polyoxin D: Effects on chitin synthetase and Morphological Development. J. Gen. Microbiol. 71, 301–309 (1972)
Bowers, B., Levin, G., Cabib, E.: Effects of Polyoxin D on chitin synthesis and septum formation in Saccharomyces cerevisiae. J. Bacteriol. 119, 564–575 (1974)
Braun, P. C., Calderone, R. A.: Chitin synthesis in Candida albicans: Comparison of yeast and hyphal forms. J. Bacteriol. 133, 1472–1477 (1978)
Cabib, E., Farkas, V.: The control of morphogenesis: An enzymatic mechanism for the initiation of septum formation in yeast. Proc. Natl. Acad. Sci., USA, 68, 2052–2056 (1971)
Cassone, A., Simonetti, N., Strippoli, V.: Ultrastructural changes in the wall during germ-tube formation from blastospores of Candida albicans. J. Gen. Microbiol. 77, 417–426 (1973)
Chattaway, F. W., Holmes, M. R., Barlow, A. J. E.: Cell wall composition of the mycelial and blastospore forms of Candida albicans. J. Gen. Microbiol. 51, 367–376 (1968)
Chattaway, F. W., Bishop, R., Holmes, M. R., Olds, F. C., Barlow, A. J. E.: Enzymic activities associated with carbohydrate synthesis in the yeast and mycelial forms of Candida albicans. J. Gen. Microbiol. 75, 97–109 (1973)
de Rousett-Hall, A., Gooday, G. W.: A kinetic study of a solubilized chitin synthetase preparation from Coprinus cinereus. J. Gen. Microbiol. 89, 146–154 (1975)
Dixon, M.: The determination of enzyme inhibitor constants. Biochem. J. 55, 170–171 (1953)
Duran, A., Bowers, B., Cabib, E.: Chitin synthetase zymogen is attached to the plasma membrane. Proc. Natl. Acad. Sci. USA 72, 3952–3955 (1975)
Duran, A., Cabib, E., Bowers, B.: Chitin synthetase distribution on the yeast plasma membrane. Science 203, 363–365 (1979)
Eggstein, M., Kreutz, F. H.: Techniques in Protein Chemistry, (Bailey, J. L. ed.), p. 340. London; Elsevier 1967
Endo, A., Kakiki, K., Misato, T.: Feedback inhibition of l-glutamine d-fructose-6-phosphate aminotransferase by uridine diphosphate N-acetylglucosamine in Neurospora crassa. J. Bacteriol. 103, 588–594 (1970)
Gancedo, J. M., Gancedo, C.: Concentrations of intermediary metabolites in yeast. Biochemie 55, 205–211 (1973)
Ghosh, S., Roseman, S.: Methods Enzymol. 5, 414–417 (1962)
Gooday, G. W.: Biosynthesis of the fungal wall-mechanisms and implications. J. Gen. Microbiol. 99, 1–11 (1977)
Gooday, G. W.: In: Developmental Mycology Vol 111 (J. E. Smith and D. R. Berry, eds.), pp. 51–77. London: Arnold 1978
Marx, J. L.: Chitin synthesis inhibitors: a new class of insecticides. Science 197, 1170–1172 (1977)
Mitani, M., Inoue, Y.: Antagonists of antifungal substance polyoxin. J. Antibiot. 21, 492–496 (1968)
McMurrough, I., Barnicki-Garcia, S.: Properties of a particulate chitin synthetase from Mucor rouxii. J. Biol. Chem. 246, 4008–4016 (1971)
Norrman, J., Giddings, T. H., Catino, E. C.: Partial purification and properties of l-glutamine: d-fructose-6-phosphate aminotransferase from zoospores of Blastocladiella emersonii. Phytochemistry 14, 1271–1274 (1975)
Peberdy, J. F., Moore, P. M.: Chitin synthase in Mortierella vinacea: properties, cellular location and synthesis in growing cultures. J. Gen. Microbiol. 90, 228–236 (1975)
Ruiz-Herrera, J., Bartncki-Garcia, S.: Proteolytic activation of chitin synthetase from Mucor rouxii. J. Gen. Microbiol. 97, 241–249 (1976)
Ryder, N. S., Peberdy, J. F.: Chitin synthase in Aspergillus nidulans: properties and proteolytic activation. J. Gen. Microbiol. 99, 69–76 (1977)
Scheckman, R., Brawley, V.: Localized deposition of chitin on the yeast cell surface in response to mating pheromone. Proc. Natl. Acad. Sci. USA 76, 645–649 (1979)
Scherwitz, C., Martin, R., Veberberg, H.: Ultrastructural investigation of the formation of Candida albicans germ-tubes and septa. Sabouraudia 16, 115–124 (1978)
Shepherd, M. G., Chiew, Y. Y., Ram, S. P., Sullivan, P. A.: Germtube induction in Candida albicans. Cand. J. Microbiol. (in press, 1980)
Stewart, P. R., Rogers, P. J.: In: Developmental mycology, Vol. 111 (J. E. Smith and D. R. Berry, eds.), pp. 164–196. London: Arnold 1978
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Chiew, Y.Y., Shepherd, M.G. & Sullivan, P.A. Regulation of chitin synthesis during germ-tube formation in Candida albicans . Arch. Microbiol. 125, 97–104 (1980). https://doi.org/10.1007/BF00403204
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DOI: https://doi.org/10.1007/BF00403204