Summary
The chloroplast (cp) genomes of Zea species are distinguished by at least four restriction fragment length (insertion/deletion) mutations. All four mutations occur in the large unique sequence region of the genome. Restriction fragments containing three of these mutations were cloned. The large and small forms of two of the mutated fragments were sequenced. This revealed 80 and 83 by insertion/deletions. The inserted/deleted segments are not parts of tandem repeats nor were they flanked by direct repeats. Two other insertion/deletion mutations were not sequenced, but their sizes were estimated to be 150 and 250 by by size fractionation on agarose gels. Use of Tripsacum pilosum and Sorghum bicolor as outgroups suggests that three of the fragment length mutations arose via deletions. The fourth could not be polarized. The three species of section Luxuriantes of Zea were identical to one another for each of the four length mutations, and they were consistantly distinguished from the taxa of section Zea by these mutations. These data support the division of Zea into the above named sections.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- bp:
-
base pairs
- kb:
-
1,000 bp
- cpDNA:
-
chloroplast DNA
- SDS:
-
sodium dodecyl sulfate
References
Anderson S, Gait MJ, Mayol L, Young IG (1980) Nucleic Acids Res 8:1731–1743
Birnboim HC, Doly J (1979) Nucleic Acids Res 7:1513–1524
Bowman CM, Bonnard G, Dyer TA (1983) Theor Appl Genet 65:247–262
Bowman CM, Dyer TA (1985) Structural variation and evolution of the chloroplast DNA of cereals. First International Congress of Plant Molecular Biology Abstracts, Athens, Georgia, pp 124
Dagert M, Ehrlich SD (1979) Genetics 6:23–28
Dale RMK, McClure BA, Houchins JP (1985) Plasmid 13:31–40
Dang LH, Pring DR (1986) Plant Mol Biol 6:119–123
Devereux J, Haeberli P, Smithies O (1984) Nucleic Acids Res 12:387–395
Doebley J, Goodman MM, Stuber CW (1984) Systematic Botany 9:203–218
Doebley J, Iltis HH (1980) Am J Bot 67:982–993
Gordon KHJ, Crouse EJ, Bohnert HJ, Hermann RG (1982) Theor Appl Genet 61:373–384
Iltis HH, Doebley J (1980) Am J Bot 67:994–1004
Koller B, Delius H (1982) EMBO J 1:995–998
Krebbers E, Steinmetz A, Bogorad L (1984) Plant Mol Biol 3:13–20
Kung SD, Zhu YS, Shen GF (1982) Theor Appl Genet 61:73–79
Larrinua IM, Muskavitch KMT, Gubbins EJ, Bogorad L (1983) Plant Mol Biol 2:129–140
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, New York
Messing J, Vieira J (1982) Genetics 19:269–276
Palmer JD (1985) Evolution of chloroplast and mitochondrial DNA in plants and algae. In: MacIntyre RJ (ed) Molecular evolutionary genetics. Plenum, New York, pp 131–240
Palmer JD (1986) Isolation and structural analysis of chloroplast DNA. In: Weissbach A, Weissbach H (eds) Plant molecular biology. Methods in enzymology, Academic, Orlando, Florida, 118:167–186
Palmer JD, Jorgensen RA, Thompson WF (1985) Genetics 109:195–213
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Theor Appl Genet 65:181–189
Quigley F, Weil JH (1985) Theor Appl Genet 9:495–503
Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA (1980) J Mol Biol 143:161–178
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Shaffer HE, Sederoff RR (1981) Anal Biochem 115:113–122
Takaiwa F, Sugiura M (1982) Nucleic Acids Res 10:2665–2676
Whitfeld PR, Bottomley W (1983) Annu Rev Plant Physiol 34:279–310
Wilkes HG (1967) Teosinte: the closest relative of maize. Bussey Inst, Harvard Univ, Cambridge, Massachusetts
Zimmer EA, Newton KJ (1982) A simple method for the isolation of high molecular weight DNA from individual maize seedlings and tissues. In: Sheridan WF (ed) Maize for biological research. Plant Molecular Biology Assoc, Charlottesville, Virginia, pp 165–168
Zurawski G, Clegg MT, Brown AHD (1984) Genetics 106:735–749
Author information
Authors and Affiliations
Additional information
Communicated by C. W. Birky, Jr.
Rights and permissions
About this article
Cite this article
Doebley, J.F., Ma, D.P. & Renfroe, W.T. Insertion/deletion mutations in the Zea chloroplast genome. Curr Genet 11, 617–624 (1987). https://doi.org/10.1007/BF00393925
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00393925