Abstract
The bud-inducing effect of the cytokinin N6-(Δ2-isopentenyl)-adenine (i6-Ade) was examined in the moss Physcomitrella patens growing in liquid culture. Under these conditions, buds could be induced on chloronemata as well as on caulonemata. By application of i6-Ade, bud-formation was accelerated in both types of tissue. The number of buds, their size and their site of development were dependent on the concentration of the cytokinin in the range of 10-7 M to 10-5 M. Moreover, the percentage of caulonema cells increased with a cytokinin concentration of 10-5 M. These results indicate that chloronema cells may also function as target cells for exogenous cytokinins. The composition of proteins from caulonemata and chloronemata of two different species (P. patens and Funaria hygrometrica), grown on solid medium were compared. No differences could be detected between the protein patterns of caulonemata and chloronemata of the same species while between the two species the differences were obvious.
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Abbreviations
- i6-Ade:
-
N6-(Δ2-isopentenyladenine)
- Da:
-
dalton
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
References
Ashton, N.W., Cove, D.J. (1977) The isolation and preliminary characterisation of auxotrophic and analogue resistant mutants of the moss, Physcomitrella patens. Mol. Gen. Genet. 154, 87–95
Ashton, N.W., Cove, D.J., Featherstone, D.R. (1979a) The isolation and physiological analysis of mutants of the moss, Physcomitrella patens, which overproduce gametophores. Planta 144, 437–442
Ashton, N.W., Grimsley, N.H., Cove, D.J. (1979b) Analysis of gametophytic development in the moss, Physcomitrella patens, using auxin and cytokinin resistant mutants. Planta 144, 427–435
Beutelmann, P., Bauer, L. (1977) Purification and identification of a cytokinin from moss callus cells. Planta 133, 215–217
Bopp, M. (1963) Development of the protonema and bud formation in mosses. J. Linn. Soc. London, Bot. 58, 305–309
Bopp, M. (1974) Action mechanism of cytokinins in mosses (a model for the effects of cytokinins). In: Plant growth substances 1973 (Proc. 8th Int. Conf. Plant Growth Subst.) pp. 934–944. Hirokawa Publishing Co. Tokyo
Bopp, M. (1981) Entwicklungsphysiologie der Moose. In: Advances in bryology, vol. 1, pp. 11–77, Schultze-Motel, W., ed. Cramer, Braunschweig
Bopp, M. (1982) How can external hormones regulate the morphogenesis of mosses? J. Hattori Bot. Lab. 53, 159–169
Bopp, M., Brandes, H. (1964) Versuche zur Protonemaentwicklung der Laubmoose. Planta 62, 116–136
Bopp, M., Jahn, H., Klein, B. (1964) Eine einfache Methode, das Substrat während der Entwicklung von Moosprotonemen zu wechseln. Rev. Bryol. Lichén. 83, 219–223
Bradford, M.M. (1976) A rapid and sensitivie method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254
Brandes, H. (1967) Die Wirkung von Kinetin auf die Entwicklung verschiedener Laubmoosarten. Rev. Bryol. Lichén. 35, 158–164
Erichsen, J., Knoop, B., Bopp, M. (1977) On the action mechanism of cytokinins in mosses: caulonema specific proteins. Planta 135, 161–168
Hahn, H., Bopp, M. (1968) A cytokinin test with high specifity. Planta 83, 115–118
Iwasa, K. (1965) Chemical control of morphogenesis in moss protonema. Plant Cell Physiol. 6, 421–429
Laemmli, U.K., Favre, M. (1973) Maturation of the head of bacteriophage T4. J. Mol. Biol. 80, 575–599
Morrissey, J.H. (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117, 301–310
Nehlsen, W. (1979) A new method for examining induction of moss buds by cytokinin. Am. J. Bot. 66, 601–603
Sood, S., Brenner, K., Bopp, M. (1978) The occurrence of caulonema-specific proteins in different moss species. Planta 138, 299–301
Sood, S., Chopra, R.N. (1973) A record preponement of bud-induction in the moss Entodon myurus. Z. Pflanzenphysiol. 69, 390–393
Szweykowska, A. (1963) Kinetin-induced formation of gametophores in dark cultures of Ceratodon purpureus. J. Exp. Bot. 14, 137–141
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Reski, R., Abel, W.O. Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isopentenyladenine. Planta 165, 354–358 (1985). https://doi.org/10.1007/BF00392232
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DOI: https://doi.org/10.1007/BF00392232