Abstract
Pith tissue of Nicotiana tabacum L. cv. “Havana 425” exhibits a gradient in its tendency to habituate for cytokinin on an auxin-containing medium at 35° C, about 10° C above the standard culture temperature. Explants of pith from below the 8th to 11th internode, counting from the bottom of the plant, rarely habituate for cytokinin; explants from above this threshold habituate rapidly. The explants must also be above a critical size, about 20–30 mg, to habituate. There was a pronounced interaction between size and position effects; the threshold position for cytokinin habituation shifted upward with decreasing explant size.
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Meins, F., Lutz, J. & Foster, R. Factors influencing the incidence of habituation for cytokinin of tobacco pith tissue in culture. Planta 150, 264–268 (1980). https://doi.org/10.1007/BF00390836
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DOI: https://doi.org/10.1007/BF00390836