Abstract
Polysomes in sieve elements of rice (Oryza sativa L.) were studied with the electron microscope. The polysomes were found on the rough endoplasmic reticulum (ER) present in immature sieve elements and also on the cisternae of aggregated ER in the parietal layer of mature, enucleate sieve elements. In the immature sieve elements the ER cisternae existed as narrow profiles while in the mature sieve elements the ER cisternae were considerably dilated and contained a fibrillar material and, occasionally, electron-opaque inclusions. In addition to the aggregated ER, single profiles of ER were found applied to the lateral walls and also the sieve plates. These cisternae also bore ribosomes and were separated from the plasmalemma by a narrow, dense space. In the mature sieve elements much of the surface of the ER membranes was covered with polysomes. The dimensions of the polysomes are described and the possibility that they contribute to the formation of the fibrillar material in the intracisternal space is discussed.
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Abbreviations
- ER:
-
endoplasmic reticulum
References
Bonnet, H.T., Newcomb, E.H. (1965) Polyribosomes and cisternal accumulations in root cells of radish. J. Cell Biol. 27, 423–432
Buvat, R (1963) Intrastructures et differentiation de cellules criblées de Cucurbita pepo. C.R. Acad. Sci. 257, 221–224
Esau, K. (1969) The phloem. In: Handbuch der Pflanzenanatomie Histologie, vol. 5, pt. 2. Zimmermann, W., Ozenda, P., Wulff, H.D., eds. Borntraeger, Berlin Stuttgart
Esau, K. (1972) Changes in the nucleus and the endoplasmic reticulum during differentiation of a sieve element in Minosa pudica. L. Ann. Bot. 36, 703–710
Esau, K. (1978) Developmental features of the primary phloem in Phaseolus vulgaris L. Ann. Bot. 42, 1–13
Esau, K., Gill, R.H. (1973) Correlations in differentiation of protophloem sieve elements of Allium cepa root. J. Ultrastruct. Res. 44, 310–328
Geitl, C., Ziegler, H. (1979) mRNA in phloem exudates? Naturwissenschaften 66, 523–524
Gunning, B.E.S., Steer, M.W. (1975) Ultrastructure and the biology of plant cells. Edward Arnold, London
Jørgensen, L.B., Behnke, H-D., Mabry, T.J. (1977) Protein-accumulating cells and dilated cisternae of the endoplasmic reticulum in three glucosinolate-containing genera: Armoracia, Capparis, Drypetes. Planta 137, 215–224
Kruatrachue, M., Evert, R.F. (1974) Structure and development of sieve elements in the leaf of Isoetes muricata. Am. J. Bot. 61, 253–266
Melaragno, J.E., Walsh, M.A. (1976) Ultrastructural features of developing sieve elements in Lemna minor L.—the protoplast. Am. J. Bot. 63, 1145–1157
Neumann, S., Wollgiehn, R. (1964) Über die Beziehungen der RNS-und Proteinsynthese zum Zellkern in Siebzellen von Vicia faba. Z. Naturforsch. 19, 1066–1071
Öpik, H. (1975) Staining of sieve tube mitochondria in coleoptiles of rice (Oryza sativa L.) with diaminobenzidine. Planta 122, 269–271
Srivastava, L.M. (1975) Structure and differentiation of sieve elements in Angiosperms and Gymmosperms. In: Phloem transport, pp. 33–62. Arnoff, S., Dainty, J., Gorham, P.R., Srivastava, L.M., Swanson, C.A., eds. NATO Advanced Study Institute Series. Plenum Press, New York London
Spurr, A.R. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43
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Oparka, K.J. Polysomes and intracisternal accumulations in enucleate sieve elements of rice (Oryza sativa L.). Planta 150, 249–254 (1980). https://doi.org/10.1007/BF00390834
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DOI: https://doi.org/10.1007/BF00390834