Abstract
A 1-min light pulse delivered to mustard seedlings (Sinapis alba L.) 60 h after sowing initiates the release of cotyledonary 5-aminolaevulinate (ALA) accumulation which continues for at least 2 h in the dark. Phytochrome (P fr) increases the rate of ALA accumulation after a 24-h red light pretreatment but is not the trigger for this release. It is shown that the rate of ALA accumulation varies with the wave-length and fluence rate of the 1-min light pulse and can be predicted from the degree of protochlorophyll-(ide) photoconversion. There is a linear correlation between the rate of ALA accumulation and the degree of protochlorophyll(ide) (PChl)→chlorophyll(ide) a (Chl a) photoconversion in etiolated seedlings. In seedlings pretreated with red light this correlation is non-linear and the rate increases more rapidly with increasing degrees of PChl→Chl a photoconversion. It is suggested that there may exist an interaction between P fr and PChl→Chl a photoconversion in controlling ALA accumulation.
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Abbreviations
- ALA:
-
5-aminolaevulinate
- Chl:
-
chlorophyll(ide)
- PChl:
-
protochlorophyll(ide)
- cp:
-
cotyledon pair
- LA:
-
laevulinate
References
Beale, S.I. (1970) The biosynthesis of δ-aminolevulinic acid in Chlorella. Plant Physiol. 45, 504–506
Belke, M. (1979) Untersuchungen zur lichtinduzierten Akkumulation von 5-Aminolävulinat in etiolierten Keimlingen von Sinapis alba L. Staatsexamensarbeit, Universität Freiburg
Björn L.O. (1975) Photobiologie. Fischer, Stuttgart, S 51
Bogorad, L. (1976) Chlorophyll biosynthesis. In: Biochemistry of chloroplasts, vol. 1, 2nd ed. Goodwin, T.W., ed. Academic Press, London
Fluhr, R., Harel, E., Klein, S., Meller, E. (1975) Control of δ-aminolevulinic acid and chlorophyll accumulation in greening maize leaves upon light-dark transitions. Plant Physiol. 56, 497–501
Gough, S.P. (1978) Light stimulated δ-aminolevulinate accumulation in levulinate treated barley seedlings. Carlsberg. Res. Commun. 43, 497–508b
Jabben, M., Masoner, M., Kasemir, H., Mohr, H. (1974) Phytochrome stimulated regeneration of protochlorophyll in cotyledons of the mustard seedling. Photochem. Photobiol. 20, 233–239
Kannangara, C.G., Gough, S.P. (1978) Biosynthesis of δ-aminolevulinate in greening barley leaves: Glutamate 1-semialdehyde aminotransferase. Carlsberg. Res. Commun. 43, 185–194
Kasemir, H., Huber, P., Mohr, H. (1976) Timing of the initial action of phytochrome with regard to protochlorophyll synthesis in the mustard seedling. Planta 132, 157–160
Klein, S., Harel, E., Ne'Eman, E., Katz, E., Meller, E. (1975) Accumulation of δ-aminolevulinic acid and its relation to chlorophyll synthesis and development of plastid structure in greening leaves. Plant Physiol. 56, 486–496
Klein, S., Katz, E., Ne'Eman, E. (1977) Induction of δ-aminolevulinic acid formation in etiolated maize leaves controlled by two light systems. Plant Physiol. 60, 335–338
Koski, V.M., French, C.S., Smith, J.H.C. (1951) The action spectrum for the transformation of protochlorophyll to chlorophyll a in normal and albino corn seedlings. Arch. Biochem. Biophys. 31, 1–17
Lohr, J.B., Friedmann, H.C. (1976) New pathway for δ-aminolevulinic acid biosynthesis: Formation from α-ketoglutaric acid by two partially purified plant enzymes. Biochem. Biophys. Res. Commun. 69, 908–913
Masoner, M., Kasemir, H. (1975) Control of chlorophyll synthesis by phytochrome. 1. The effect of phytochrome on the formation of 5-amino-levulinate in mustard seedlings. Planta 126, 111–117
Miller, G.W., Denney, A., Wood, J.K., Welkie, G.W. (1979) Light induced delta-aminolevulinic acid in dark-grown barley seedlings. Plant and Cell Physiol. 20, 131–143
Mohr, H. (1966) Untersuchungen zur Phytochrom-induzierten Photomorphogenese des Senfkeimlings. Z. Pflanzenphysiol. 54, 63–68
Schiff, J.A., Epstein, U.T. (1966) The replicative aspect of chloroplast continuity in Euglena. In: Biochemistry of chloroplasts, Goodwin, T.W., ed., Academic Press, London
Ziegler, R., Egle, K. (1965) Zur quantitativen Analyse der Chloroplastenpigmente. 1. Kritische Überprüfung der spektralphotometrischen Chlorophyll-Bestimmung. Beitr. Biol. Pflanzen 41, 11–37
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Ford, M.J., Kasemir, H. Correlation between 5-aminolaevulinate accumulation and protochlorophyll photoconversion. Planta 150, 206–210 (1980). https://doi.org/10.1007/BF00390827
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DOI: https://doi.org/10.1007/BF00390827