Summary
A number of inhibitors of DNA-, RNA-, and protein synthesis were applied to prothallia of Anemia phyllitidis, and their effects were investigated with regard to cell division and inhibition of antheridium formation (after induction by gibberellin A3). All compounds tested cause a significant inhibition of growth accompanied by teratologies typical for each inhibitor. Only 5-bromouracil proved to be inactive, even at a concentration of 8×10-4m.
None of the inhibitors blocked the induction of the biplanar growth form in continuous light.
With the exception of 5-bromodeoxyuridine and 5-iododeoxyuridine all the antimetabolites investigated cause a significant lag in antheridium formation. The result of an analysis of this inhibition on the basis of the “critical cell number” (Schraudolf, 1966a) demonstrates that this time lag is clearly a direct consequence of the retardation of cell division caused by the inhibitors. There is no inhibition of the induction process proper. So therefore it appears highly improbable that antheridium induction in Anemia by gibberellins is based on a specific gene activation in the sense of Jacob and Monod.
The results presented in this paper demonstrate that the production of a time lag in the realisation of a process of differentiation or morphogenesis is not sufficient evidence to permit definite conclusions to be drawn about the basic mechanism of induction; the effect of inhibition of cell division caused by the inhibitors must be excluded or at least taken in to account.
The possible function of gibberellins as “realisators” of already present but inactive m-RNA is discussed.
5-Bromo- and 5-iododeoxyuridine do not cause a time lag in antheridium formation; both inhibitors, however, cause a deformation of the cell pattern of the antheridia. The degree of this deformation depends on inhibitor concentration as well as on exposure time. Probably a competition exists between these halogensubstituted pyrimidines and thymidine for incorporation into DNA.
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Literatur
Bennett Jr., L.L., D. Smithers, C. Teague, H. T. Baker, and P. Sutts: Some effects of 4-aminopyrazolo-(3,4-d)-pyrimidine on purine metabolism. Biochem. Pharmacol. 11, 81–92 (1962).
Booth, B. A. and A. C. Sartorelli: 4-Aminopyrazolo-(3,4-d)-pyrimidine: An inhibitor of the synthesis of purines and proteins in Ehrlich ascites cells. J. biol. Chem. 236, 203–206 (1961).
Brockman, R. W., and E. P. Anderson: Pyrimidine analogues. In: Metabolic Inhibitors (eds. R. M. Hochster and J. H. Quastel), p. 239–285. New York: Academic Press 1963.
Brown, J. A. M.: Effect of thymidine analogues on reproductive morphogenesis in Arabidopsis thalliana (L.) Heynh. Nature (Lond.) 196, 51–53 (1962).
Hotta, Y., and S. Osawa: Controle of differentiation in the fern gametophyte by amino acid analogs and 8-azagunine. Exp. Cell Res. 15, 85–94 (1958).
——, and T. Sakaki: Ribonucleic acid differentiation of the gametophyte of a polypodiaceous fern. Develop. Biol. 1, 65–78 (1959).
Karlson, P.: Morphogenese und Metamorphose der Insekten. Coll. Ges. Physiol. Chem. 13, 101–126 (1962).
Kasten, F. H., F. F. Strasser, and M. Turner: Nucleolar and cytoplasmatic ribonucleic acid inhibition by excess thymidine. Nature (Lond.) 207, 161–164 (1965).
Kressel, M.: Antheridien-und Archegonienbildung bei Microlepia speluncae (L.) Moore. Z. Pflanzenphysiol. 53, 366–368 (1965).
MacComb, A.J.: The stability and movement of gibberellic acid in pea seedlings. Ann. Bot. 28, 669–687 (1964).
Marks, P. A., E. R. Burka, E. M. Conconi, W. Perl, and R. A. Rifkind: Polyribosome dissociation and formation in intact reticulocytes with conservation of messenger ribonucleic acid. Proc. nat. Acad. Sci. (Wash.) 53, 1437–1443 (1965).
Mohr, H.: Die Steuerung der Entwicklung durch Licht am Beispiel der Farngametophyten. Ber. dtsch. bot. Ges. 78, 54–68 (1965).
Monroy, A., R. Maggio and A. M. Rinaldi: Experimentally induced activation of the ribosome of the unfertilized sea urchin egg. Proc. nat. Acad. Sci. (Wash.) 54, 107–111 (1965).
Naef, U.: On dark germination and antheridium formation in Anemia phyllitidis. Physiol. plantacum 19, 1079–1088 (1966).
Nakazawa, S., and N. Tanno: Concentration gradients of RNA in fern protonema in relation to m-RNA. Naturwissenschaften 52, 457 (1965).
Overbeek, J. Van: Plant hormons and regulators. Science 152, 721–731 (1966).
Pilet, P.: Les phytohormones de croissance, p. 241. Paris: Masson & Cie. 1961.
Pringle, R.B.: Chemical structure of antheridogen A, a specific inducer of the male sex organ in certain fern species. Science 133, 173–187 (1961).
Rhagavan, V.: Action of purine and pyrimidine analogs on the growth and differentiation of the gametophytes of the fern Asplenium nidus. Amer. J. Bot. 52, 900–910 (1965).
Schraudolf, H.: Die Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien: I. Auslösung der Antheridienbildung und Dunkelkeimung bei Schizaeaceen durch Gibberellinsäure. Biol. Zbl. 81, 731–740 (1962).
—: Einige Beobachtungen zur Entwicklung der Antheridien von Anemia phyllitidis. Flora (Jena) 153, 282–290 (1963).
—: Relative activity of the gibberellins in the antheridium induction in Anemia phyllitidis. Nature (Lond.) 201 98–99 (1964).
—: Die Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien: II. Analyse der Wechselbeziehung zwischen Gibberellinkonzentration, Antheridienbildung und physiologischem Alter der Prothalliumzellen von Anemia phyllitidis. Planta (Berl.) 68, 335–352 (1966a).
—: Die Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien: IV. Die Wirkung von unterschiedlichen Gibberellinen und von Allogibberinsäure auf die Auslösung der Antheridienbildung bei Anemia phyllitidis und einigen Polypodiaceen. Plant and Cell Physiol 7, 277–289 (1966b).
Schraudolf, H.: Wirkung von IES, Cumarin und sogenannten “Antigibberellinen” auf die Auslösung der Antheridienbildung in Anemia phyllitidis durch Gibberelline. Z. Pflanzenphysiol. im Druck (1966c).
Singer, F.M., and P. Leder: Messenger-RNA: An evaluation. Ann. Rev. Biochem. 35, 195–230 (1966).
Smith, H.H., and B.H. Kugelman: Incorporation of tritiated base analogs into plant chromosomes. Rad. Res. 14, 504 (1961).
Spirin, A. S., N. V. Bellitzina i M. A. Aitkhozin: Informationnye RNK v rammen embriogeneze. Zh. Obshch. Biol. 25, 321–338 (1964)
——: Informationye ribonukleinovye v rammen embriogeneze. Usp. sovrem. Biol. 59, 187–204 (1965).
Varner, J. E., and G. Ram Chandra: Hormonal controle and enzyme synthesis in barley endosperm. Proc. nat. Acad. Sci. (Wash.) 52, 100–106 (1964).
Werz, G.: primärvorgänge bei der Realisation der Morphogenese von Acetabularia. Planta (Berl.) 69, 53–57 (1966).
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Schraudolf, H. Wirkung von hemmstoffen der DNS-, RNS- und proteinsynthese auf wachstum und antheridienbildung in prothallien von Anemia phyllitidis L.. Planta 74, 123–147 (1967). https://doi.org/10.1007/BF00388325
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DOI: https://doi.org/10.1007/BF00388325