Summary
The electrical resistance across the wall of proximal tubules of the rat kidney has been determined in situ. Two different experimental approaches were used. First, the resistance between lumen and interstitium, the so-called effective resistance (R eff) according to cable analysis, was measured. The mean values, as obtained by different methods, scattered between 25 and 100 kω, the most probable value being 30–40 kω. Second, the passive voltage attenuation along the tubular axis, as described by the length constant (λ) was determined. Applying different methods a value of λ=86 μ was derived. With the simple model of cable analysis these results lead to a specific transepithelial resistance of 4.9–5.7 ωcm2, implying an apparent electrical diameter of the tubular lumen of 26–30 μ. This diameter is larger than the geometrical one. The value for the specific transephithelial resistance agrees well with results of recent measurements of unidirectional ion fluxes.
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Baines, A. D., P. P. Leyssac, and C. W. Gottschalk: Proximal tubular volume and inulin clearance in non-diuretic rats. Internat. Congr. Nephrol., Vol. II, p. 152. Washington 1966.
Baumann, K., H. Holzgreve, F. Kolb, R. Peters, G. Rumrich u. K. J. Ullrich: Unidirektionale Flüsse für Na24, K42, Ca45, Cl38, Br82 und J131 im proximalen Konvolut der Rattenniere. Pflügers Arch. ges. Physiol. 289, R 77 (1966). Ausführliche Veröffentlichung in Pflügers Arch. ges. Physiol. (in Vorbereitung).
Brunner, F. P., F. C. Rector, and D. W. Seldin: Mechanism of glomerulotubular balance. II: Regulation of proximal tubular reabsorption by tubular volume, as studied by stopped-flow microperfusion. J. clin. Invest. 45, 603 (1966).
Diamond, J. R.: The mechanism of solute transport by the gall bladder. J. Physiol. (Lond.) 161, 474 (1962).
Fatt, P., and B. Katz: The electrical properties of crustacean muscle fibres. J. Physiol. (Lond.) 120, 171 (1953).
Feldtkeller, R.: Einführung in die Vierpoltheorie der elektrischen Nachrichtentechnik. Leipzig: Hirzel 1948.
Frank, K., and M. C. Becker: Microelectrodes for recording and stimulation. In: Physical Techniques in Biological Research, ed. by W. L. Nastuk, Vol. V, p. 22. Chapt. 2. New York: Academic Press 1964.
Frömter, E., u. U. Hegel: Transtubuläre Potentialdifferenzen an proximalen und distalen Tubuli der Rattenniere. Pflügers Arch. ges. Physiol. 291, 107 (1966).
-- T. Wick u. U. Hegel: Untersuchungen zur Ausspritzmethode für die Lokalisation der Mikroelektrodenspitze bei transtubulären Potentialmessungen an der Ratte. Pflügers Arch. ges. Physiol. (im Druck).
Gertz, K. H.: Transtubuläre Natriumchloridflüsse und Permeabilität für Nichtelektrolyte im proximalen und distalen Konvolut der Rattenniere. Pflügers Arch. ges. Physiol. 276, 336 (1963).
Giebisch, G., R.M. Klose, G. Malnic, W. J. Sullivan, and E. E. Windhager: Sodium movement across single perfused proximal tubules of rat kidneys. J. gen. Physiol. 47, 1175 (1964).
Hegel, U., u. E. Frömter: Erfahrungen mit der Öltropfenmethode zur Lokalisation der Mikroelektrodenspitze bei transtubulären Potentialmessungen an der Rattenniere. Pflügers Arch. ges. Physiol. 291, 121 (1966).
Loewenstein, W. R.: Permeability of membrane junctions. Ann. N.Y. Acad. Sci. 137, 441 (1966).
Stevens, Ch. E.: Transport of sodium and chloride by the isolated rumen epithelium. Amer. J. Physiol. 206, 1099 (1964).
Ullrich, K. J., K. Baumann, K. Loeschke, G. Rumrich, and H. Stolte: Micropuncture experiments with saluretic sulfonamides. Ann. N. Y. Acad. Sci. (in press).
Ussing, H. H., and E. E. Windhager: Nature of shunt path and active Natransport path through frog skin epithelium. Acta physiol. scand. 61, 484 (1964).
—, and K. Zehran: Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta physiol. scand. 23, 110 (1951).
Wiederholt, M., K. Hierholzer, E. E. Windhager u. G. Giebisch: Einfluß unterschiedlicher Perfusionsraten auf die proximale Flüssigkeitsresorption (Mikroperfusionsuntersuchungen an der Rattenniere). Pflügers Arch. ges. Physiol. 289, R 68 (1966). Die ausführliche Veröffentlichung befindet sich unter dem Titel “Effect of different rates of perfusion on fluid reabsorption in single proximal tubules of rat kidney” im J. Amer. Physiol. (im Druck).
Windhager, E. E., E. L. Boulpaep, and G. Giebisch: Electrophysiological studies on single nephrons. Internat. Congr. Nephrol., Vol. I, p. 68. Washington 1966.
—, and G. Giebisch: Comparison of short circuit current and net water movement in single perfused proximal tubules of rat kidneys. Nature (Lond.) 191, 1205 (1961).
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Hegel, U., Frömter, E. & Wick, T. Der elektrische Wandwiderstand des proximalen Konvolutes der Rattenniere. Pflügers Archiv 294, 274–290 (1967). https://doi.org/10.1007/BF00363113
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DOI: https://doi.org/10.1007/BF00363113