Summary
To elucidate biochemical features leading to p-hydroxyphenyl-rich lignin in gymnosperm reaction wood the activities of the following five enzymes involved in the biosynthesis of p-hydroxyphenyl lignin were compared in reaction and opposite woods: phenylalanine ammonialyase (EC 4.3.1.5), cinnamate 4-hydroxylase (EC 1.14.13.11), p-hydroxycinnamate: CoA ligase (EC 6.2.1.12), cinnamyl alcohol dehydrogenase (EC 1.1.1.-) and peroxidase (EC 1.11.1.7). The enzyme activities in the reaction woods of Thuja orientalis and Metasequoia glyptostroboides were remarkably higher than those in the opposite woods, reflecting the higher contents of p-hydroxyphenyl lignin in reaction wood.
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References
Bland, D. E. 1961: The chemistry of reaction wood. Part 3. Holzforschung 15: 102–106
Kutsuki, H.; Higuchi, T. 1981a: Activities of some enzymes of lignin formation in reaction wood of Thuja orientalis, Metasequoia glyptostroboides and Robinia pseudoacacia. Planta 152: 365–368
Kutsuki, H.; Nakatsubo, F.; Higuchi, T. 1981b: A new synthesis of coniferaldehyde. Mokuzai Gakkaishi 27: 520–522
Kutsuki, H.; Shimada, M.; Higuchi, T. 1981c: Distribution and roles of p-hydroxycinnamate: CoA ligase in lignin biosynthesis. Phytochemistry (in press)
Kutsuki, H.; Shimada, M.; Higuchi, T. 1982: Regulatory role of cinnamyl alcohol dehydrogenase in the formation of guaiacyl and syringyl lignins. Phytochemistry 21: 19–23
Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. 1951: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275
Morohoshi, N.; Sakakibara, A. 1971a: The chemical composition of reaction wood. 1. Mokuzai Gakkaishi 17: 393–399
Morohoshi, N.; Sakakibara, A. 1971b: The chemical composition of reaction wood. 2. Mokuzai Gakkaishi 17: 400–404
Russell, D. W. 1971: The metabolism of aromatic compounds in higher plants. J. Biol. Chem. 264: 3870–3878
Sarkanen, K. V. 1971: Precursors and their polymerization. In: Sarkanen, K. V. and Ludwig, C. H. (Eds.): Lignins. pp. 95–163. New York: Wiley Interscience
Tomimura, Y.; Yokoi, T.; Terashima, N. 1980: Heterogeneity in formation of lignin. 5. Mokuzai Gakkaishi 26: 37–42
Yasuda, S.; Sàkakibara, A. 1975: The chemical composition of lignin from compression wood. Mokuzai Gakkaishi 21: 363–369
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This work was partly supported by the Grant-in-Aid for Scientific Research (548047) from the Ministry of Education, Science and Culture of Japan. We thank the Ministry of Education for the support
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Kutsuki, H., Higuchi, T. Activities of some enzymes of lignin formation in reaction wood of Thuja orientalis and Metasequoia glyptostroboides 2. Wood Sci. Technol. 16, 287–291 (1982). https://doi.org/10.1007/BF00353155
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DOI: https://doi.org/10.1007/BF00353155