Summary
Transcription of the 2.6 kbp psbK-psbI-trnG cluster in tobacco chloroplasts has been studied. This cluster contains, in linear sequence, the genes encoding two low-molecular-mass polypeptides, K and I, of photosystem II (psbK and psbI, respectively), and tRNAGly (UCC) (trnG). Northern blot hybridization revealed that the largest transcript (2.6 kb) hybridizes to psbK, psbI and trnG, but not to the following trnR-UCU. Ten other transcripts ranging from 0.1 to 1.3 kb were also detected. Three of these transcripts overlap the divergent transcript arising from trnS-GCU located on the opposite DNA strand. S1 mapping and primer extension experiments showed that these multiple transcripts comprise eight distinct 5′ ends. By in vitro capping assays two of them were determined to be transcriptional initiation sites; one is located 163 bp upstream of psbK and the other is 6 bp upstream of trnG. The 3′ ends of transcripts were determined by S1 mapping; one lies between psbI and trnG and the other is at the end of trnG. The presence of dual promoters of trnG is discussed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Deno H, Sugiura M (1983) Nucleic Acids Res 11:8407–8414
Deno H, Sugiura M (1984) Proc Natl Acad Sci USA 81:405–408
Gray JC (1987) In: Amesz J (ed) Photosynthesis. Elsevier, Amsterdam, pp 319–342
Herrmann RG, Westhoff P, Alt J, Tittgen J, Nelson N (1985) In: Van Vloten-Doting L, Groot GSP, Hall T (eds) Molecular form and function of the plant genome. Plenum, New York, pp 233–256
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun CR, Meng BY, Li Y, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194
Howe CJ, Barker RF, Bowman CM, Dyer TA (1988) Curr Genet 13:343–349
Ikeuchi M, Inoue Y (1988) FEBS Lett 241:99–104
Kennell JC, Pring DR (1989) Mol Gen Genet 216:16–24
Meng BY, Tanaka M, Wakasugi T, Ohme M, Shinozaki K, Sugiura M (1988) Curr Genet 14:395–400
Murata N, Miyao M, Hayashida N, Hidaka T, Sugiura M (1988) FEBS Lett 235:283–288
Neuhaus H, Link G (1990) Curr Genet 18:377–383
Sexton TB, Jones JT, Mullet JE (1990 a) Curr Genet 17:445–454
Sexton TB, Christopher DA, Mullet JE (1990 b) EMBO J 9:4485–4494
Shinozaki K, Sugiura M (1982) Gene 20:91–102
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Shinozaki-Yamaguchi K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Stern DB, Gruissem W (1987) Cell 51:1145–1157
Sugiura M (1989) Annu Rev Cell Biol 5:51–70
Sugiura M, Shinozaki K, Zaita N, Kusuda M, Kumano M (1986) Plant Sci 44:211–216
Tanaka M, Obokata J, Chunwongse J, Shinozaki K, Sugiura M (1987) Mol Gen Genet 209:427–431
Webber AN, Packman L, Chapman DJ, Barber J, Gray JC (1989) FEBS Lett 242:259–262
Yao WB, Meng BY, Tanaka M, Sugiura M (1989) Nucleic Acids Res 17:9583–9591
Author information
Authors and Affiliations
Additional information
Communicated by H. Kössel
Rights and permissions
About this article
Cite this article
Meng, BY., Wakasugi, T. & Sugiura, M. Two promoters within the psbK-psbI-trnG gene cluster in tobacco chloroplast DNA. Curr Genet 20, 259–264 (1991). https://doi.org/10.1007/BF00326241
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00326241