Summary
The effects of short- and long-term exposure to a range in concentration of sea salts on the kinetics of NH sup+inf4 uptake by Spartina alterniflora were examined in a laboratory culture experiment. Long-term exposure to increasing salinity up to 50 g/L resulted in a progressive increase in the apparent Km but did not significantly affect Vmax (mean Vmax=4.23±1.97 μmole·g−1·h−1). The apparent Km increased in a nonlinear fashion from a mean of 2.66±1.10 μmole/L at a salinity of 5 g/L to a mean of 17.56±4.10 μmole/L at a salinity of 50 g/L. These results suggest that the long-term effect of exposure to total salt concentrations within the range 5–50 g/L was a competitive inhibition of NH sup+inf4 uptake in S. alterniflora. No significant NH sup+inf4 uptake was observed in S. alterniflora exposed to 65 g/L sea salts. Short-term exposure to rapid changes in salinity significantly affected both Vmax and Km. Reduction of solution salinity from 35 to 5 g/L did not change Vmax but reduced Km by 71%. However, exposing plants grown at 5 g/L salinity to 35 resulted in an decrease in Vmax of approximately 50%. Exposure of plants grown at 35 g/L to a total sea salt concentration of 50 g/L for 48h completely inhibited uptake of NH sup+inf4 . For both experiments, increasing salinity led to an increase in the apparent Km similar to that found in response to long-term exposure. Our data are consistent with a conceptual model of changes in the productivity of S. alterniflora in the salt marsh as a function of environmental modification of NH sup+inf4 uptake kinetics.
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References
Adams DA (1963) Factors influencing vascular plant zonation in North Carolina salt marshes. Ecology 44: 445–456
Bard Y (1967) Non-Linear Parameters Estimation and Programming. SHARE Program Library, IBM 360D-13.6.003. IBM New York Scientific Center Publication, New York
Bradley PM, Dunn EL (1989) Effect of sulfide on the growth of three salt marsh halophytes of the Southeastern US. Am J Bot 76: 1707–1713
Bradley PM, Morris JT (1990) Influence of oxygen and sulfide concentration on nitrogen uptake kinetics in Spartina alterniflora. Ecology 71: 282–287
Broome SW, Woodhouse WW, Seneca ED (1975) The relationship of mineral nutrients to growth of Spartina alterniflora in North Carolina. II. The effects of N, P, and Fe fertilizers. Soil Sci Soc Am Proc 39: 301–307
Cavalieri AJ, Huang AHC (1979) Evaluation of proline accumulation in the adaptation of diverse species of marsh halophytes to the saline environment. Am J Bot 66: 307–312
Cavalieri AJ, Huang AHC (1981) Accumulation of proline and glycinebetaine in Spartina alterniflora Loisel. in response to NaCl and nitrogen in the salt marsh. Oecologia 49: 224–228
Chalmers AG (1982) Soil dynamics and the productivity of Spartina alterniflora. In: Kennedy VS (ed) Estuarine Comparisons. Acedemic Press, New York pp 231–242
Classen N, Barber SA (1974) A method for characterizing the relation between nutrient concentration flux into roots of intact plants. Plant Physiol 54: 564–568
DeLaune RD, Smith CJ, Patrick WH Jr (1983) Relationships of marsh elevation, redox potential, and sulfide to Spartina alterniflora productivity. J Soil Sci Soc Am 47: 930–935
Epstein E, Hagen CE (1952) A kinetic study of the absorption of akalai cations by barley roots. Plant Physiol 27: 457–474
Gallagher JL (1975) Effect of an ammonium nitrate pulse on the growth and elemental composition of natural stands of Spartina alterniflora and Juncus roemerianus. Am J Bot 62: 644–648
Giurgevich JR, Dunn EL (1979) Seasonal patterns of CO2 and water vapor exchange of the tall and short height forms of Spartina alterniflora Loisel in a Georgia salt marsh. Oecologia 43: 139–156
Haines BL, Dunn EL (1976) Growth and resource allocation responses of Spartina alterniflora Loisel. to three levels of NH4-N, Fe, and NaCl in solution culture. Bot Gaz 137: 224–230
Haines EB, Chalmer AG, Hanson RB, Sherr B (1977) Nitrogen pools and fluxes in a Georgia salt marsh. In: Wiley J (ed) Estuarine Processes Volume II. Academic Press New York pp 241–254
Hopkinson CS, Schubauer JP (1984) Static and dynamic aspects of nitrogen cycling in the salt marsh graminoid S. alterniflora. Ecology 65: 961–969
King GM, Klug MJ, Wiegert RG, Chalmers AG (1982) Relation of soil water movement and sulfide concentration to Spartina alterniflora productivity in a Georgia salt marsh. Science 218: 61–63
Linthurst RA, Seneca ED (1981) Aeration, nitrogen and salinity as determinants of Spartina alterniflora Loisel growth response. Estuaries 4: 53–63
Longstreth DJ, Strain BR (1977) Effects of salinity and illumination on photosynthesis and water balance of Spartina alterniflora Loise. Oecologia 31: 191–199
Mendelssohn IA (1979) Nitrogen metabolism in the height forms of Spartina alterniflora in North Carolina. Ecology 60: 574–584
Mendelssohn IA, McKee KL (1988) Spartina alterniflora die-back in Louisiana: Time-course investigation of soil waterlogging effects. J Ecol 76: 509–521
Montgomery DC (1984) Design and Analysis of Experiments. John Wiley & Sons New York pp 69–70
Morris JT (1980) The Nitrogen Uptake Kinetics Of Spartina alterniflora in culture. Ecology 61: 1114–1121
Morris JT (1984) Effects of oxygen and salinity on ammonia uptake by Spartina alterniflora (loisel) and Spartina patens (aiton). J Exp Mar Biol Ecol 78: 87–98
Morris JT, Dacey JWH (1984) Effects of O2 on ammonium uptake and root respiration by Spartina alterniflora. Amer J Bot 71: 979–985
Nestler J (1977) Interstitial salinity as a cause of ecophenic variation in Spartina alterniflora. Est Coast Mar Sci 5: 707–714
Neter J, Wasserman W, Kutner MH (1985) Applied Linear Statistical Models. Richard D. Irwin, Inc. Homewood, Illinois pp 574–579
Nye PH, Tinker PB (1977) Chapter 5: The uptake properties of the root system in solution. In: Solute Movement in the Soil Root System. Univ Berkley Press Los Angeles pp 104–111
Patrick WH Jr, DeLaune RD (1976) Nitrogen and phosphorus utilization by Spartina alterniflora in a salt marsh in Barataria Bay, Louisiana. Estuarine Coast Mar Sci 4: 59–64
Phleger PF (1971) Effect of salinity on growth of a salt marsh grass. Ecology 52: 908–911
Rains DW, Epstein E (1967) Preferential absorption of potassium by leaf tissue of the mangrove, Avicennia marina: an aspect of halophytic competence in coping with salt. Austr J Biol Sci 20: 847–857
SAS INSTITUTE Inc. (1985) SAS User's Guide: Statistics. SAS Institute Inc. Cary, N.C.
Shiflet TN (1963) Major ecological factors controlling plant communities in Louisiana marshes. J Range Manag 16: 231–235
Smart RM, Barko JW (1980) Nitrogen nutrition and salinity tolerance of Distichlis spicata and Spartina alterniflora. Ecology 61: 630–638
Smart RM (1982) Distribution and environmental control of productivity and growth forms of Spartina alterniflora (Loisel.). in Sen DN, Raipurohit KS (eds.) Contributions to the Ecology of Halophytes. W. Junk Publishers, The Hague, Netherlands pp 127–142
Solarzano L (1969) Determination of ammonium in natural waters by the phenolhydrochlorite method. Limnol Oceanogr 14: 799–801
Tromp J (1962) Interactions in the absorption of ammonium, potassium and sodium ions by wheat roots. Acta Bot Neerlandica 11: 147–192
Valiela I, Teal JM (1974) Nutrient limitation in salt marsh vegetation, Reimold RJ and Queen WH (eds) Ecology of Halophytes. Acedemic Press. New York. pp 547–563
Valiela I, Teal JM, Deuser WG (1978) The nature of growth forms in the salt marsh grass Spartina alterniflora. Am Nat 112: 461–470
Webb JW (1983) Soil water salinity variations and their effects on Spartina alterniflora. Contr Mar Sci 26: 1–13
Whiting GJ, McKellar HN Jr, Kjerfve B, Spurrier JD (1987) Nitrogen exchange between a southeastern USA salt marsh ecosystem and the coastal ocean. Marine Biol 95: 173–182
Wilkinson GN (1961) Statistical estimations in enzyme kinetics. Biochem J 80: 324–332
Woodhouse WW Jr, Seneca ED, Broome SW (1974) Propagation of Spartina alterniflora for substrate stabilization and salt marsh development. US Army Corps of Engineers, Coastal Engineering Research Center Ft. Belvoir, VA. Technical Memorandum 46. p155
Woodwell GM, Hall CAS, Whitney DE, Houghton RA (1979) The Flax Pond ecosystem study: exchanges of inorganic nitrogen between an estuarine marsh and Long Island Sound. Ecology 60: 695–702
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Bradley, P.M., Morris, J.T. The influence of salinity on the kinetics of NH sup+inf4 uptake in Spartina alterniflora . Oecologia 85, 375–380 (1991). https://doi.org/10.1007/BF00320613
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DOI: https://doi.org/10.1007/BF00320613