Summary
A case of fatal acute encephalitis due to Coxsackie B1 virus is described. Confirmation of Coxsackie B virus as the etiological agent of encephalitis was based on identification of the virus antigen in formalin-fixed paraffin-embedded tissue sections. In the past, the diagnosis was obtained by serological studies of peripheral blood and viral isolation. This is the first report in which indirect immunofluorescent and immunoper-oxidase methods using rabbit antiserum raised against Coxsackie B types 1–6 was utilized in determining the etiology of encephalitis. It must be emphasized that these methods can be used both on biopsy or autopsy specimens, even retrospectively.
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Booss J, Esiri MM (1986) Viral encephalitis: pathology, diagnosis and management. Blackwell Scientific Publications,Oxford, pp 143–147
Chommaitree T, Menegus MA, Powell KR (1982) The clinical relevance of CSF viral culture: a 2-year experience with aseptic meningitis in Rochester, New York. J Am Med Assoc 247: 1843–1847
Dalldorf G, Gifford R (1952) Adaption of group B Coxsackie virus to adult mouse pancreas. J Exp Med 96:491–497
Delaney TB, Fukunaga DM (1958) Myocarditis in a newborn infant with encephalomeningitis due to Coxsackie virus group B. type 4. N Engl J Med 259:234–236
Eilard T, Kyllerman M, Wennerblom I, Olofsson OE, Lycke E (1974) An outbreak of Coxsackie virus type B2 among neonates in an obstetrical ward. Acta Pediatr Scand 63:103–107
Fechner RE, Smith MG, Middelkamp JN (1963) Coxsackie B virus infection of the newborn. Am J Pathol 42:493–505
Hondo R, Kurata T, Sato S, Oda A, Aoyama Y (1982) Enzymatic treatment of formalin-fixed and paraffin-embedded speciments for detection of antigen of herpes simplex, varicella zoster and human cytomegalovirus. Jpn J Exp Med 52:17–25
Huang S, Minassian H, More JD (1976) Application of immunofluorescent staining of paraffin sections improved by trypsin digestion. Lab Invest 35:383–390
Javett SN, Hyemann S, Mundel B, Pepler WJ, Lurie HI, Gear J, Measroch V, Kirsch Z (1956) Myocarditis in the newborn infant: a study of an outbreak associated with Coxsackie group B virus infection in a maternity home in Johannesburg. J Pediatr 48:1–22
Johnson RT (1982) Viral infections of the nervous system. Raven Press, New York, pp 96–98
Kibrick S, Benirschke K (1958) Severe generalized disease (encephalohepatomyocarditis) occurring in the newborn period and due to infection with Coxsackie virus, group B evidence for intrauterine infection with this agent. Pediatrics 22:857–875
Klapper PE, Bailey AS, Longson M, Davies-Jones GAB (1984) Meinigoencephalitis caused by Coxsackievirus group B type 2: diagnosis confirmed by measuring intrathecal antibody. J Infect 8:227–231
Lee FK, Visintine AN, Takey Y, Whitley R, Dannenbager J, Nahimia AJ (1981) Rapid detection of viruses in biopsy or autopsy specimens by pseudoreplica method of electron microscopy. Am J Surg Pathol 5:565–572
Marier R, Rodriguez W, Chloupek RJ, Brandt CD, Kim HW Baltimore RS, Parker CL, Artenstein MS (1975) Coxsackie B5 infection and aseptic meningitis in neonates and children. Am J Dis Child 129:321–325
McLean DM, Donohue WI, Snelling CE, Wyllie JC (1961) Coxsackie B5 virus as a cause of neonatal encephalitis and myocarditis. Can Med Assoc J 85:1046–1048
Moossy J, Geer JC (1960) Encephalomyelitis, myocarditis and adrenal cortical necrosis in Coxsackie B3 virus infection: distribution of central nervous system lesions. Arch Pathol 70:614–622
Newton WA, Hosier DM (1958) Coxsackie infection in infants and children: focal and disseminated; fatal and nonfatal. Am J Pathol 34:587
Pappenheimer AM, Kunz LJ, Richardson S (1951) Passage of Coxsackie virus (Connecticut-5 strain) in adult mice with production of pancreatic disease. J Exp Med 94:45–64
Rotbart HA, Levin MJ, Villarreal LP, Tracy SM, Semler BL, Wimmer E (1985) Factors affecting the detection of enteroviruses in cerebrospinal fluid with Coxsackie B3 and poliovirus 1 cDNA probes. J Clin Microbiol 22:220–224
Sata T, Kurata T, Aoyama Y, Sakaguchi M, Yamanouchi K, Takeda K (1986) Analysis of viral antigens in giant cells of measles pneumonia by immunoperoxidase method. Virchows Arch [A] 410:133–138
Smith R, Woddall JP, Whitney E, Deibel R, Cross MA (1974) Powassan virus infection: a report of three human cases of encephalitis. Am J Dis Child 127:691–693
Ujevich MM, Jaffee R (1980) Pancreatic islet cell damage: its occurrence in neonatal Coxsackievirus encephalomyocarditis. Arch Pathol Lab Med 104:438–441
Walker DH, Chain BG (1981) Specific diagnosis of Rock Mountain spotted fever using fixed, paraffin-embedded tissue by immunofluorescence. J Infect Dis 137:206–209
Wenner H (1982) The enteroviruses: recent advances. Yale J Biol Med 55:277–282
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Presented in part at the sixty-third Annual Meeting of the American Association of Neuropathologists, Seattle, Washington, June 11–14, 1987
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Kamei, S., Hersch, S.M., Kurata, T. et al. Coxsackie B antigen in the central nervous system of a patient with fatal acute encephalitis: immunohistochemical studies of formalin-fixed paraffin-embedded tissue. Acta Neuropathol 80, 216–221 (1990). https://doi.org/10.1007/BF00308928
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DOI: https://doi.org/10.1007/BF00308928