Summary
Frozen sections of primary and metastatic human renal cell carcinoma (RCC) were analyzed for the expression of endogeneous binding sites for carbohydrates. Fluorescent neoglycoproteins, carrying chemically linked carbohydrate residues on bovine serum albumin as a carrier protein, were applied to 44 primary tumor specimens. In the majority of specimens, accessible binding sites with specificity for maltose and N-acetylgalactosamine were detected. In specimens of normal kidney no specific binding of carbohydrate ligands was observed under these experimental conditions. Specimens of both the primary tumor and a metastasis were available in 10 cases. When the expression of specific binding sites of primary tumors and metastases was compared, the respective patterns were similar with no clear gain or loss of certain lectins in the metastases. We conclude that binding sites with specificity for maltose and N-acetylgalactosamine are present on human RCC and their corresponding metastases.
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Arthus MF, Bergeron M, Scriver C (1982) Topology of membrane exposure in the renal cortex slice: studies of glutathione and maltose cleavage. Biochim Biophys Acta 692:371
Bardosi A, Bardosi L, Hendrys M, Wosgien B, Gabius HJ (1990) Spatial differences in endogeneous lectin expression within the cellular organization of the human heart. Am J Anat 188:409
deKernion JB, Ramming KP, Smith RB (1978) The natural history of metastatic renal cell carcinoma: a computer analysis. J Urol 120:148
Eder M (1984) Die Metastasierung: Fakten und Probleme aus humanpathologischer Sicht. Verh Dtsch Ges Pathol 68:1
Gabius HJ (1991) Detection and function of mammalian lectins with emphasis on membrane lectins. Biochim Biophy Acta 1071:1
Gabius HJ, Bardosi A (1991) Neoglycoproteins as tools in glycohistochemistry. Progr Histochem Cytochem 22:1
Gabius HJ, Grote T, Gabius S, Brinck U, Tietze LF (1991) Neoglycoprotein binding to colorectal tumour cells: comparison between primary and secondary lesions. Virchows Arch [A] 419:217
Gabius S, Schirrmacher V, Franz H, Joshi SS, Gabius HJ (1991) Analysis of cell surface receptor expression by neoglycoenzyme binding and adhesion to plastic-immobilized neoglycoproteins for related low and high metastatic cell lines of murine origin. Int J Cancer 46:500
Glaves D, Gabius HJ, Weiss L (1989) Site-associated expression of endogenous tumor lectins. Int J Cancer 44:506
Hart I, Easty D (1991) Identification of genes controlling metastatic behaviour. Br J Cancer 63:9
Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin peroxidase complex (ABC) in immunperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577
Heicappell R, Ackermann R (1990) Rationale for immunotherapy of renal cell carcinoma. Urol Res 18:357
Kerbel RS, Waghorne C, Korezak B, Lagarde A Breitman ML (1988) Clonal dominace of primary tumours by metastaic cells: genetic analysis and biological implications. Cancer Surv 7:597
Kieda C, Roche AC, Delmotte F, Monsigny M (1979) Lymphocyte membrane lectins. FEBS Lett 99:329
Kieda C, Monsigny M (1986) Involvement of membrane sugar receptors and membrane glycoconjugates in the adhession of 3LL cell subpopulation to cultured pulmonary cells. Invasion Metastasis 6:347
Kolb H, Kolb-Bachhofen V, Kolb HA (1978) Possible mechanism of entrapment of neuraminidase-treated lymphocytes in the liver. Cell Immunol 40:457
Lotan R, Raz A (1988) Endogenecous lectins as mediators of tumor cell adhesion. J Cell Biochem 37:107
Monsigny M, Kieda C, Roche AC (1983) Membrane glycoproteins, glycolipids and membrane lectins as recognition signals in normal and malignant cells. Biol Cell 47:95
Nicolson GL (1988) Organ specificity of tumor metastasis: role of preferential adhesion, invasion and growth of malignant cell at specific secondary sites. Cancer Metastasis Rev 7:143
Paget S (1889) Ohe distribution of secondary cancer growth in cancer of the breast. Lancet I:571
Pataroyo M, Makgoba MW (1989) Leukocyte adhesion to cells in immune and inflammatory responses. Lancet I:1139
Raz A, Ben-Ze'ev A (1987) Cell-contact and-architecture of malignant cells and their relationship to metastasis. Cancer Metastasis Rev 6:3
Sharon N, Lis H (1989) Lectins as cell recognition molecules. Science 246:227
Tahara Y, Fukuda M, Yamamoto Y, Noma Y, Yamato E, Cha T, Yoneda H, Ikegami H, Hirota M, Shima K (1990) Metabolism of intraveneously administered maltose in renal tubules in humans. Am J Clin Nutr 52:689
Tannenbaum M (1983) Surgical and histopathology of renal tumors. Semin Oncol 10:385
Tosaka A, Ohya K, Yamada K, Ohashi H, Kitahara S, Sekine H, Takehara Y, Oka K (1990) Incidence and properties of renal masses and asymptomatic renal cell carcinoma detected by abdominal ultrasonography. J Urol 144:1097
Vidal-Vanaclocha F, Barbera-Guillem E, Weiss L, Glaves D, Gabius HJ (1990) Quantitation of endogeneous lectin expression in 3LL tumors, growing subcutaneosly and in the kidney of mice. Int J Cancer 46:908
Yednock TA, Rosen SD (1989) Lymphocyte homing. Adv Immunol 44:313
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Buszello, H., Gabius, HJ., Ackermann, R. et al. Detection of endogenous receptors for carbohydrate ligands in primary and metastatic human renal cell carcinoma. Urol. Res. 21, 293–299 (1993). https://doi.org/10.1007/BF00307715
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DOI: https://doi.org/10.1007/BF00307715