Summary
Gynogenesis could be an efficient way for producing inbred lines in commercial fish species. Gamma-irradiation of sperm gives haploid embryos that all die without hatching; in the present study, we optimized heat treatment of the eggs, in order to produce high rates of diploid gynogenetics. When the eggs are heated to 26 °C for 20 min after 25 min of development, 80% of the embryos hatch, and all the resulting fry are diploid; nevertheless, high mortalities are recorded until feeding start. The monosex female constitution of gynogenetic offspring confirms the female homogamety in the rainbow trout.
When the eggs are treated with the same heat shock 25 min after a fertilization with functional sperm, alltriploid populations are obtained; their survival until feeding start is not different from the control.
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Literature
Billard, R. (1974): L'insémination artificielle chez la truite Salmo gairdneri Richardson. 4: Effet des ions K et Na sur la conservation de la fertilité des gamètes. Bull. Fr. Pisc. 256, 88–100
Cherfas, N.B.; Truweller, K. (1978): Investigation of radiationinduced gynogenesis in carp (Cyprinus carpio L.): 3: Gynogenetic offspring analysis by biochemical markers (in Russian). Genetika 14, 599–604
Chevassus, B. (1976): Variabilité et héritabilité des performances de croissance chez la truite arc-en-ciel (Salmo gairdneri Richardson) Ann. Génét. Sél. Anim. 8, 273–283
Chourrout, D.; Chevassus, B.; Hérioux, F. (1980): Analysis of a Hertwig effect in the rainbow trout (Salmo gairdneri R.) after fertilization with gamma irradiated sperm. Reprod. Nutr. Develop. 20, 719–726
Chourrout, D. (1980): Thermal induction of diploid gynogenesis and triploidy in the rainbow trout (Salmo gairdneri R.). Reprod. Nutr. Develop. 20, 726–733
Guyomard, R. (1981): Electrophoretic variation in four French populations of domesticated rainbow trout (Salmo gairdneri). Can. J. Genet. Cytol. 23, 33–47
Jaylet, A. (1972): Recherches sur diverses modifications expérimentales de l'assortiment chromosomique chez le triton Pleurodeles waltlii: remaniement du caryotype, tétraploidie, gynogenèse. Thèse Doctrat es Sciences naturelles. No. 466, Toulouse: Université Paul Sabatier
Kincaid, H.L. (1978): Crossbreeding rainbow trout strains and inbred lines. Fisheries and Wildlife Research. Report of the US Department of Interior
Kincaid, H.L.; Bridges, W.R.; Von Limbach, B. (1977): Three generations of selection for growth rate in fall-spawning rainbow trout. Trans. Am. Fish. Soc. 106, 621–628
Klupp, R. (1979): Genetic variance for growth in rainbow trout (Salmo gairdneri). Aquaculture 18, 123–134
Moav, R.; Wohlfarth, G. (1976): Two-way selection for growth rate in the common carp (Cyprinus carpio L.). Genetics 82, 83–101
Nagy, A.; Rajki, K.; Horvath, L.; Csanyi, V. (1978): Investigation on carp, Cyprinus carpio, gynogenesis. J. Fish Biol. 13, 215–224
Okada, H.; Matumoto, H.; Yamazaki, F. (1979): Functional masculinization of genetic females in rainbow trout. J. Jpn. Soc. Sci. Fish. 45, 413–419
Purdom, C.E. (1969): Radiation-induced gynogenesis and androgenesis in fish. Heredity 24, 431–434
Purdom, C.E.; Thompson, D.; Dando, P.R. (1976): Genetic analysis of enzyme polymorphisms in plaice (Pleuronectes platessa). Heredity 37, 193–206
Refstie, T.; Steine, T.A. (1978): Selection experiments with salmon. 3: Genetic and environmental sources of variation in length and weight of Atlantic salmon in the freshwater phase. Aquaculture 14, 221–234
Richards, C.M.; Nace, G.W. (1978): Gynogenetic and hormonal sex reversal used in tests of the XX-XY hypothesis of sex determination in Rana pipiens. Growth 42, 319–331
Ryman, N. (1972): An attempt to estimate the magnitude of additive genetic variation in the guppy-fish, Lebistes reticulatus. Hereditas 71, 237–244
Stanley, J.G.; Sneed, K.E. (1974): Artificial gynogenesis and its application in genetics and selective breeding of fishes. In: Early life history of fish (ed. Blaxter, J.H.S.) Berlin, Heidelberg, New York: Springer
Stanley, J.G. (1976): Female homogamety in grass carp (Ctenopharyngodon idella) determined by gynogenesis. J. Fish. Res. Bd. Can. 33, 1373–1374
Thoorgard, G.H.; Jazwin, M.E.; Stier, A.R. (1981): Polyploidy induced by heat shock in rainbow trout. Trans. Am. Fish. Soc. 110, 546–549
Volpe, E.P.; Dasgupta, S. (1962): Gynogenetics diploids of mutant leopard frog. J. Exp. Zool. 151, 287–301
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Chourrout, D., Quillet, E. Induced gynogenesis in the rainbow trout: Sex and survival of progenies production of all-triploid populations. Theoret. Appl. Genetics 63, 201–205 (1982). https://doi.org/10.1007/BF00303993
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DOI: https://doi.org/10.1007/BF00303993