Summary
Urease-induced crystallisation was studied in different human urine samples after urease incubation. The studies were performed using the Coulter counter technique, which enables determination of the number and size of particles in a solution and calculation of the total particle volume. The crystallization took place in three consecutive but overlapping steps: (1) nucleation, (2) growth and (3) aggregation. The maximal number of particles obtained in the different samples varied little, but there was a great variation in particle size and total particle volume. The variation in particle size appeared to be mainly due to differences in particle growth, a factor that might be of importance for stone formation.
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References
Adamthwaite DN (1983) Particulate activity in urine. Br J Urol 55:55:89
Baumann JM (1985) Can the formation of calcium oxalate stones be explained by crystallisation processes in urine? Urol Res 13:267
Carruthers BM, Copp DM, McIntosh HW (1964) Diurnal variation in urinary excretion of calcium and phosphate and its relation to blood levels. J Lab Clin Med 63:959
Fleisch H (1978) Inhibitors and promoters of stone formation. Kidney Int 13:361
Grenabo L, Hedelin H, Pettersson S (1986) The inhibitory effect of human urine on urease induced crystallisation in vitro. J Urol 135:416
Griffith DP, Musher DM, Itin C (1976) Urease, the primary cause of infection induced urinary stones. Invest Urol 13:346
Hedelin H, Grenabo L, Pettersson S (1985) Urease induced crystallisation in synthetic urine. J Urol 133:529
Hedelin H, Grenabo L, Pettersson S (1986) The effects of urease in undiluted human urine. J Urol 136:743
Higuchi WI (1964) Rate of solute transport out of emulsion droplets in micron size range. J Pharm Sci 53:405
Higuchi WI, Lau PK (1962) Experimental method for studying growth rates of particles in aqueous media. J Pharm Sci 51:1081
Hugosson J, Hedelin H, Grenabo L, Pettersson S (1987) Effects of plasma proteins and breast milk on crystallisation of magnesium ammonium phosphate and calcium phosphate. In: Martelli A, Buli P, Marchesini B (eds) Inhibitors of crystallisation in renal lithiasis and their clinical application. Acta Medica, Bologna, p 225
Resnick MI, Boyce WH (1979) Aethiological theories of renal lithiasis. In: Wickham JEA (ed) Urinary calculus disease. Churchill Livingstone, London, p 1
Robertson WG (1969) A method of measuring calcium crystalluria. Clin Chim Acta 43:31
Robertson WG, Peacock M, Nordin BEC (1969) Calcium crystalluria in recurrent renal-stone-formers. Lancet II:21
Ryall RL, Ryall RG (1981) Interpretation of particle growth and aggregation patterns obtained from the Coulter counter: a simple theoretical model. Invest Urol 18(5):396
Ryall RL, Hibberd CM, Marshall VR (1985) A method for studying inhibitory activity in whole urine. Urol Res 13:285
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Wang, Y.H., Grenabo, L., Hedelin, H. et al. Studies of urease-induced crystallisation in undiluted human urine using the Coulter counter technique. Urol. Res. 19, 171–175 (1991). https://doi.org/10.1007/BF00303744
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DOI: https://doi.org/10.1007/BF00303744