Summary
Nucellar callus lines were established from two orange cultivars (‘Nucellar Shamouti’, ‘Shamouti Landau’), three mandarin cultivars (‘Murcott’, ‘Dancy’, ‘Ponkan’) one grapefruit cultivar (‘Duncan’) and sour orange (Citrus aurantium). These callus lines were initiated from in vitro cultured ovules of young fruits and maintained an embryogenic capacity. The plating efficiencies of protoplasts derived from these calli, as well as those of protoplasts from lemon (cv. ‘Villafranca’) nucellar callus were differentially affected by the maceration enzymes and by the sugars used as osmotic stabilizers. Plants with normal morphological features were regenerated from cultured protoplasts derived from each of the nucellar callus lines. The establishment of eight new protoplast systems in Citrus paves the way for cell genetics studies and for novel breeding approaches in these economically important orchard trees.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literature
Barrett, H.C.; Rhodes, A.M. (1976): A numerical taxonomic study of affinity relationships in cultivated Citrus and its close relatives. Syst. Bot. 1, 105–136
Bourgin, J.P.; Chupeau, Y.; Missonier, C. (1979): Plant regeneration from mesophyll protoplasts of several Nicotiana species. Physiol. Plant. 45, 288–292
Cameron, J.W.; Frost, H.B. (1968): Genetic breeding and nucellar embryony. In: The Citrus Industry (eds. Reuther, W.; Batchelor, L.D.; Webber, H.J.), pp. 325–370. Berkeley: Univ. of California, Div. of Agric. Sci.
Galun, E.; Aviv, D.; Raveh, D.; Vardi, A.; Zelcer, A. (1977): Protoplasts in studies of cell genetics and morphogenesis. In: Proceedings in Life Science (eds. Reinhard, E.; Alfermann, A.W.), pp. 301–312. Berling, Heidelberg, New York: Springer
Kochba, J.; Spiegel-Roy, P. (1976): Improvement of plant production in Citrus by tissue culture. Plant Propagator 22, 11–12
Kochba, J.; Spiegel-Roy, P.; Safran, H. (1972): Adventive plants from ovules and ovule-parts in Citrus. Planta 106, 237–245
Melchers, G. (1980): The future. In: Perspectives in Plant Cell and Tissue Culture (eds. Vasil, I.K.), Int. Rev. Cytol. 11B, 241–253
Murashige, T.; Tucker, D.P.H. (1969): Growth factor requirements of Citrus tissue culture. In: Proc. First. Int. Citrus Symp., vol. 3 (ed. Chapman, H.D.), pp. 1155–1161. Riverside: Univers. California
Power, J.B.; Frearson, E.M.; George, D.; Evans, P.K.; Berry, S.F.; Hayward, C.; Cocking, E.C. (1976): The isolation culture and regeneration of leaf protoplasts in the genus Petunia. Plant Sci. Lett. 7, 51–55
Singh, B.D.; Harvey, B.L. (1975): Effect of different 2,4-D concentrations on the cytogenetic behaviour of plant cells cultured in vitro. Biol. Plant. 17, 167–174
Vardi, A. (1977): Isolation of protoplasts in Citrus. Proc. Int. Soc. Citriculture. 2, 575–578
Vardi, A. (1978): Studies on isolation and regeneration of orange protoplasts. In: Proc. Int. Symp. Plant Cell Culture (eds. Alfermann, A.W.; Reinhard, E.), pp. 234–242. Munich: Gesell. Strahl. Umweltforsch.
Vardi, A.; Raveh, D. (1976): Cross-feeder experiments between tobacco and orange protoplasts. Z. Pflanzenphysiol. 78, 350–359
Vardi, A.; Spiegel-Roy, P.; Galun, E. (1975): Citrus cell culture isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos. Plant Sci. Lett. 4, 231–236
Vardi, A.: Spiegel-Roy P. (1978): Citrus breeding, taxonomy and the species problems. Proc. Int. Soc. Citriculture 3, 51–57
Vasil, I.K.; Vasil, V. (1980): Isolation and culture of protoplasts. In: Perspective in Plant Cell and Tissue Culture (ed. Vasil. I.K). Int. Rev. Cytol. 11b, 1–19
Zelcer, A.; Aviv, D.; Galun, E. (1978): Interspecific transfer of cytoplasmic male sterility by fusion between protoplasts of normal Nicotiana sylvestris and X-ray irradiated protoplasts of male-sterile N. tabacum. Z. Pflanzenphysiol. 90, 397–407
Author information
Authors and Affiliations
Additional information
Communicated by P. Maliga
Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. No. E-290, 1981 series
Rights and permissions
About this article
Cite this article
Vardi, A., Spiegel-Roy, P. & Galun, E. Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species. Theoret. Appl. Genetics 62, 171–176 (1982). https://doi.org/10.1007/BF00293354
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00293354