Abstract
Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 107 following PEG-induced uptake of Sma1 linearised pABD1 plasmid. DNA-treated protoplasts were cultured in agarose droplets, and protoplast-derived transformants selected on 80 μg ml−1 kanamycin. Transformed tissues maintained on this level of antibiotic expressed APH(3′)II activity, and contained DNA that hybridised to a probe with the APH(3′)II gene.
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Abbreviations
- APH(3′)II:
-
aminoglycoside phosphotransferase
- PEG:
-
polyethylene glycol
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
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Chen, W.H., Gartland, K.M.A., Davey, M.R. et al. Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene. Plant Cell Reports 6, 297–301 (1987). https://doi.org/10.1007/BF00272003
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DOI: https://doi.org/10.1007/BF00272003