Summary
pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.
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Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, H.C., Heynecker, H.K., Boyer, H.W.: Construction and characterisation of a new cloning vehicle II. A multipurpose cloning system. Gene 2, 95–113 (1977)
Curtiss III, R., Pereira, D.A., Hsu, J.C., Hull, S.C., Clark, J.E., Maturin, L.J., Sr., Goldschmidt, R., Moddy, R., Inoue, M., Alexander, L.: In: Recombinant molecules, Miles International Symposium Series (eds. Beers, R.F. Bassett, E.G.), pp. 45–56. New York: Raven Press, 1977
Davies, J., Jacob, F.: Genetic mapping of the regulator and operator genes of the lac operon. J. Mol. Biol. 36, 413–417 (1968)
Gronenborn, B., Messing, J.: Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites. Nature 272, 375–377 (1978)
Langley, K.E., Fowler, A.V., Zabin, I.: Amino acid sequence of β-galactosidase IV. Sequence of an α-complementing cyanogen bromide peptide, residues 3 to 92. J. Biol. Chem. 250, 2587–2592 (1975b)
Langley, K.E., Villarejo, M.R., Fowler, A.V., Zamenhof, P.J., Zabin, I.: Molecular basis of β-galactosidase α-complementation. Proc. Natl. Acad. Sci. U.S.A. 72, 1254–1257 (1975a)
Lederberg, J., Lederberg, E.M.: Replica plating and indirect selection of bacterial mutants. J. Bacteriol. 63, 399–406 (1952)
Mandel, M., Higa, A.: Calcium-dependent bacteriophage DNA infection. J. Mol. Biol. 53, 159–162 (1970)
Messing, J., Gronenborn, B., Müller-Hill, B., Hofschneider, P.H.: filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro. Proc. Natl. Acad. Sci. U.S.A. 74, 3642–3646 (1977)
Miller, J.H.: Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory 1972
Sutcliffe, J.G.: pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide long. Nucleic Acids Res. 5, 2721–2728 (1978)
Ullmann, A., Jacob, F., Monod, J.: Characterisation by in vitro complementation of a peptide corresponding to an operatorproximal segment of the β-galactosidase structural gene of Escherichia coli. J. Mol. Biol. 24, 339–343 (1967)
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Communicated by E. Bautz
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Rüther, U. Construction and properties of a new cloning vehicle, allowing direct sereening for recombinant plasmids. Molec. Gen. Genet. 178, 475–477 (1980). https://doi.org/10.1007/BF00270503
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DOI: https://doi.org/10.1007/BF00270503