Summary
We constructed transformants of B. subtilis 168 which acquired genes for site-specific restriction endonucleases. These endonucleases originated from various strains of B. subtilis and were classified into five groups based on the specificity of the sequences recognized by the enzymes. We examined the loci of genes for site-specific restriction endonucleases belonging to different groups: hsrE determined Endo.R.Bsu1231(I), hsrB Endo.R.Bsu1247(I), hsrR Endo.R.BsuR and hsrC Endo.R.Bsu1247(II). One gene, hsrE, was located between sacA and purA by transduction crosses with phage PBS1, and another gene, hsrB, between hsrE and purA.
Genes hsrR and hsrC had been suggested to be allelic or closely linked by previous studies with transformation. We located hsrR and hsrC between purB and tre.
Our previous observation and this study show that B. subtilis 168 has at least three independent loci on the chromosome for four genes for site-specific restriction endonucleases in addition to the locus for the original restriction activity (Bsu168-specific restriction) of strain 168.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Betlach M, Hershfield V, Chow L, Brown W, Goodman H, Boyer HW (1976) A restriction endonuclease analysis of the bacterial plasmid controlling the EcoRI restriction and modification of DNA. Fed Proc 35:2037–2043
Bigger CH, Murray K, Murray NE (1973) Recognition sequence of a restriction enzyme. Nature New Biology 244:7–10
Bron S, Murray K (1975) Restriction and modification in B. subtilis. Nucleotide sequence recognized by restriction endonuclease R.BsuR from strain R. Mol Gen Genet 143:25–33
Bron S, Murray K, Trautner TA (1975) Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R. Mol Gen Genet 143:13–23
Chang S, Cohen SN (1977) In vivo site-specific genetic recombination promoted by the EcoRI restriction endonuclease. Proc Natl Acad Sci USA 74:4811–4815
Dubnau D (1971) Genetic mapping of Bacillus subtilis. In: Grossman L, Moldave K (ed) Methods in enzymology, vol 21 part D. Nucleic acids. Academic Press, New York and London, pp 430–438
Dubnau D, Davidoff-Abelson R, Smith I (1969) Transformation and transduction in Bacillus subtilis: Evidence for separate modes of recombinant formation. J Mol Biol 45:155–179
Dubnau D, Goldthwaite C, Smith I, Marmur J (1967) Genetic mapping in Bacillus subtilis. J Mol Biol 27:163–185
Garro AJ, Leffert H, Marmur J (1970) Genetic mapping of a defective bacteriophage on the chromosome of Bacillus subtilis 168. J Virol 6:340–343
Henner DJ, Hoch JA (1980) The Bacillus subtilis Chromosome. Microbiol Rev 44:57–82
Ikawa S, Shibata T, Ando T (1976) The site-specific deoxyribonuclease from Bacillus pumilus (Endonuclease R.Bpu1387). J Biochem (Tokyo) 80:1457–1460
Ikawa S, Shibata T, Ando T, Saito H (1979) Host-controlled modification and restriction in Bacillus subtilis. Bsu168-system and BsuR-system in B. subtilis 168. Mol Gen Genet 170:123–127
Ikawa S, Shibata T, Ando T, Saito H (1980) Genetic studies on site-specific endodeoxyribonucleases in Bacillus subtilis: Multiple modification and restriction systems in transformants of Bacillus subtilis 168. Mol Gen Genet 177:359–368
Kejzlarova-Lapesant J, Harford N, Lepesant JA, Dedoner R (1975) Revised genetic map for Bacillus subtilis 168. In: Gerhardt P, Costilow RN, Sadoff HL (eds) Spore VI. American Society for Microbiology, Washington, D.C., pp 592–595
Kelly Jr TJ, Smith HO (1970) A restriction enzyme from Hemophilus influenzae. II Base sequence of the recognition site. J Mol Biol 51:393–409
Lederberg S (1957) Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria. Virology 3:496–513
Lees ND, Welker NE (1973) Restriction and modification of bacteriophage in Bacillus stearothermophilus. J Virol 11:606–609
Lepesant JA, Lepesant Kejzlarova J, Pascal M, Kunst F, Billault A, Dedonder R (1974) Identification of the structural gene of levansucrase in Bacillus subtilis Marburg. Mol Gen Genet 128:213–221
Lepesant-Kejlarova J, Lepesant JA, Walle J, Billault A, Dedonder R (1975) Revision of the linkage map of Bacillus subtilis 168: Indications for circularity of the chromosome. J Bacteriol 121:823–834
Miller CA, Cohen SN (1978) Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid. Proc Natl Acad Sci USA 75:1265–1269
Roberts RJ (1980) Restriction and modification enzymes and their recognition sequences. Gene 8:329–343
Saito H, Shibata T, Ando T (1979) Mapping of genes determining nonpermissiveness and host-specific restriction to bacteriophages in Bacillus subtilis Marburg. Mol Gen Genet 170:117–122
Seaman E, Tarmy E, Marmur J (1964) Inducible phages of Bacillus subtilis. Biochemistry 3:607–613
Shibata T, Ando T (1974) Host controlled modification and restriction in Bacillus subtilis. Mol Gen Genet 131:275–280
Shibata T, Ando T (1975) In vitro modification and restriction of phage ϕ105C DNA with Bacillus subtilis N cell-free extract. Mol Gen Genet 138:269–279
Shibata T, Ando T (1976) The restriction endonucleases in Bacillus amyloliquefaciens N strain. Substrate specificities. Biochim Biophys Acta 442:184–196
Shibata T, Saito H (1973) Repair of ultraviolet-induced DNA damage in the subcellular systems of Bacillus subtilis. Mutat Res 20:159–173
Shibata T, Ikawa S, Kim C, Ando T (1976) Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains. J Bacteriol 128:473–476
Shibata T, Ikawa S, Komatsu Y, Ando T, Saito H (1979) Introduction of host-controlled modification and restriction systems of Bacillus subtilis TAM1247 into Bacillus subtilis 168. J Bacteriol 139:308–310
Smith HO, Wilcox KW (1970) A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol 51:379–391
Thurm P, Garro AJ (1975) Isolation and characterization of prophage mutants of the defective Bacillus subtilis bacteriophage PBSX. J Virol 16:184–191
Trautner TA, Pawlek B, Bron S, Anagnostopoulos C (1974) Restriction and modification in B. subtilis. Biological aspects. Mol Gen Genet 131:181–191
Warner FD, Kitos GA, Romano MP, Hemphill HE (1977) Characterization of SPβ: a temperate bacteriophage from Bacillus subtilis 168M. Can J Microbiol 23:45–51
Wilson GA, Young FE (1975) Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H. J Mol Biol 97:123–125
Wood WB (1966) Host specificity of DNA produced by Escherichia coli: Bacterial mutations affecting the restriction and modification of DNA. J Mol Biol 16:118–133
Young FE, Wilson GA (1975) Chromosomal map of Bacillus subtilis. In: Gerhardt P, Costilow RN, Sadoff HL (eds) Spore VI. American Society for Microbiology, Washington DC, pp 596–614
Zahler SA, Korman RZ, Rosenthal R, Hemphill HE (1977) Bacillus subtilis Bacteriophage SP β: Localization of the prophage attachment site, and specialized transduction. J Bacteriol 129:556–558
Author information
Authors and Affiliations
Additional information
Communicated by W. Arber
Rights and permissions
About this article
Cite this article
Ikawa, S., Shibata, T., Matsumoto, K. et al. Chromosomal loci of genes controlling site-specific restriction endonucleases of Bacillus subtilis . Molec. Gen. Genet. 183, 1–6 (1981). https://doi.org/10.1007/BF00270129
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00270129