Abstract
Callus cultures of Choisya ternata have been prepared by different strategies: aggregate clones, subclones and protoclones obtained from well-established strains; protoclones obtained from mesophyll tissue; cultures transformed by Agrobacterium tumefaciens. All of them show high variability in their dihydrofuroquinoline alkaloid production. As compared to the alkaloid content of the whole plant, one alkaloid (platydesminium) could be obtained in higher amounts in some lines, but it was impossible to get high-balfourodinium accumulating lines. Moreover balfourodinium-producing capacities were lower in transformed cells as compared to those of normal cell lines.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- MS:
-
Murashige and Skoog (1962)
- B5:
-
Gamborg B5 (1968) medium
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphtaleneacetic acid
- BA:
-
N6-benzyladenine
- EtOH:
-
ethanol
- d.w.:
-
dry weight
- sd:
-
standard deviation
References
Crèche J, Guiller J, Andreu F, Gras M, Chénieux J-C, Rideau M (1985) Phytochemistry 26: 1947–1953
Constabel F, Rambold S, Chatson KB, Kurz WGW, Kutney JP (1981) Plant Cell Reports 1: 3–9
Dougall DK, Johnson JM, Whitten GH (1980) Planta 149: 292–297
Eilert V, De Luca V, Kurz WGW, Constabel F (1987) Plant Cell Reports 6: 271–274
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50: 151–158
Jullien M (1980) in Chaussat R, Bigot C (eds). La multiplication végétative des plantes supérieures, Gauthier-Villars, Paris, 161–190
Montagu M, Rideau M, Levillain P, Chénieux J-C (1980) Planta medica 38: 50–53
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–49
Oksman-Caldentey KM, Strauss A (1986) Planta medica 52: 6–12
Otten LABM, Schilperoort RA (1978) Biochim. Biophys. Acta 527: 497–500
Payne J, Rhodes MJC, Robins RJ (1987) Planta medica: 367–372
Pétiard V, Baubault C, Bariaud A, Hutin M, Courtois D (1986) In: Neumann KH, Barz W, Reinhard E (eds). Primary and secondary metabolism of plant cell cultures, Springer, Berlin, Heidelberg, New-York, Tokyo, 133–140
Rideau M, Verchère C, Hibon P, Chénieux J-C, Maupas P, Viel C (1979) Phytochemistry 20: 353–355
Trémouillaux-Guiller J, Andreu F, Crèche J, Chénieux J-C Rideau M (1987) Plant Cell Reports 6: 375–178
Author information
Authors and Affiliations
Additional information
Communicated by W. Barz
Rights and permissions
About this article
Cite this article
Trémouillaux-Guiller, J., Kodja, H., Andreu, F. et al. Variability in tissue cultures of Choisya ternata. III comparing alkaloid production in cell lines obtained by various strategies. Plant Cell Reports 7, 456–458 (1988). https://doi.org/10.1007/BF00269537
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269537