Abstract
A new protocol for regenerating plants from cultured type I callus of the maize (Zea mays L.) inbred Pa91 includes growing the callus on medium containing 3.5 mg/l (15.5 μM) of the cytokinin 6-benzylaminopurine (6BA) for 3 to 6 d and then moving the callus to medium containing no growth regulators (H medium) for an additional 15 to 21 d, where the plants actually develop. The number of plants regenerated from the 6BA treated callus was 113% to 148% greater than the number of plants produced from callus placed directly on H medium. This increased plant regeneration induced by 6BA seemed to maximize the number of plants regenerated from a gram of callus and was slightly affected by callus age or prior treatment of callus with AgNO3. Exposure to 6BA for 9 d greatly reduced shoot and root development, and longer exposures totally prevented root formation. This inhibition of root formation could be reversed only slightly by naphthaleneacetic acid. The data indicate that high concentrations of 6BA are effective for increasing plant regeneration from maize callus cultures when short exposure times are used. This procedure has also been effective for regenerating many plants from the inbreds H99 and Mo17.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- 6BA:
-
6-Benzylaminopurine
- IAA:
-
indole-3-acetic acid
- NAA:
-
Naphthaleneacetic Acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- dicamba:
-
3,6-dichloro-o-anisic acid
- GA3 :
-
Gibberellic acid
- gfw:
-
gram fresh weight
Literature
Armstrong CL, Green CE (1985) Planta 164:207–214
Beyer EM Jr (1976) Plant Physiol 58:268–271
Chang Y-F (1983) Plant Cell Reports 2:183–185
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Sci Sin 18:659–668
Christianson ML (1987) In: Green CE, Somers DA, Hackett WP, Biesboer DD (eds) Plant tissue and cell culture, pp 45–55
Duncan DR, Williams ME, Zehr BE, Widholm JM (1985) Planta 165:322–332
Duncan DR, Widholm JM (1987a) Plant Physiol 83:705–708
Duncan DR, Widholm JM (1987b) Plant Physiol 83 suppl: 35
Fahey JW, Reed JN, Readdy TL, Pace CM (1986) Plant Cell Reports 5:35–38
Green CE, Armstrong CL, Anderson PC (1983) In: Downey K, Voellmy RW, Ahmad F, Schultz J (eds) Molecular genetics of plants and animals, pp 147–157
Green CE, Phillips RL (1975) Crop Sci 15:417–421
Harms CT, Lorz H, Potrykus I (1976) Z. Pflanzenzuchtg 77:347–351
Kamo KK, Becwar MR, Hodges TK (1985) Bot Gaz 146:327–334
Lowe K, Taylor DB, Ryan P, Paterson KE (1985) Plant Sci 41:125–132
Lu C, Vasil V, Vasil KJ (1983) Theor Appl Genet 66:285–289
Malmberg R, Messing J, Sussex I (1984) Molecular biology of plants, Cold Spring Harbor, NY pp 23–25
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Novak FJ, Dolezelova M, Nesticky M, Piovarci A (1983) Maydica XXVIII:381–390
Radojevic L (1985) J Plant Physiol 119:435–441
Santos MA, Torne JM, Blanco JL (1984) Plant Sci Lett 33:309–315
Skoog F, Miller CO (1957) Symp Soc Exptl Biol 11:118–130
Songstad DD, Duncan DR, Widholm JM (1988) Plant Cell Reports 7:262–265
Torne JM, Santos MA, Pons A, Blanco M (1980) Plant Sci Lett 17:339–344
Torne JM, Santos MA, Blanco JL (1984) Plant Sci Lett 33:317–325
Vasil V, Lu C-Y, Vasil IK (1983) Amer J Bot 70L951-954
Vasil V, Vasil IK (1984) In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, pp 36–42
Author information
Authors and Affiliations
Additional information
Communicated by E. D. Earle
Rights and permissions
About this article
Cite this article
Duncan, D.R., Widholm, J.M. Improved plant regeneration from maize callus cultures using 6-benzylaminopurine. Plant Cell Reports 7, 452–455 (1988). https://doi.org/10.1007/BF00269536
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269536