Abstract
Protoplasts isolated from embryogenic suspension cultures of Citrus mitis were cultured in a medium without any plant growth substances. Somatic embryos developed directly from protoplasts without an obvious intervening callus phase. As many as 1,800 somatic embryos developed from 4 ml of protoplast suspension (density 2×106/ml) cultured for 35 days. Upon transferring the embryoids to medium with 1 mgl−1 GA3, they developed into plant-lets. Rooted plantlets were obtained in 3 months after protoplast isolation.
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Abbreviations
- BAP:
-
Benzylaminopurine
- GA3 :
-
Gibberellic acid
- MT:
-
Murashige and Tucker medium (1969)
- FDA:
-
Fluorescein diacetate
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Communicated by I. K. Vasil
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Sim, GE., Loh, CS. & Goh, CJ. Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco. Plant Cell Reports 7, 418–420 (1988). https://doi.org/10.1007/BF00269527
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DOI: https://doi.org/10.1007/BF00269527