Abstract
Wheat (Triticum aestivum L. cv Chinese Spring) protoplasts were isolated from immature embryos or embryogenic calli (3–4 weeks of culture on MS medium with 32 mg/1 dicamba) and cultured in R2 medium containing 2 mg/1 2,4-D by the nurse culture methods originally developed for rice protoplasts (Kyozuka et al. 1987). Protoplasts isolated from embryogenic calli started to divide within 3–5 days and formed colonies at frequencies up to 2% after 3–4 weeks of culture, while protoplasts isolated from immature embryos formed colonies at much lower frequency (less than 0.1%). Some of these colonies were “embryogenic”, and they appeared at a frequency of approximately 0.5% of colonies formed when callus-derived protoplasts were used. From two of those “embryogenic” colonies, calli were regenerated and albino shoots and roots were obtained.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abdullah R, Cocking EC, Thompson JA (1986) Bio/Technology 4: 1087–1090
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Sci Sin 16: 659–668
Chuang CC, Ouyang TW, Chia H, Chou SM, Ching CK (1978) In: Proceeding of Symposium on Plant Tissue Culture, Peking, Scientific Press, pp 51–56
Felsenburg T, Feldman M, Galun E (1987) Theor Appl Genet 74: 802–810
Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Plant Tissue Culture Lett 2: 74–75
Harms CT, Potrykus I (1978) Theor Appl Genet 53: 57–63
Hayashi Y, Kyozuka J, Shimamoto K (1988) Mol Gen Genet (in press)
Imbrie-Milligan CW, Hodges TK (1986) Planta 168: 395–401
Kao KN, Michayluk MK (1975) Planta 126: 105–110
Kyozuka J, Hayashi Y, Shimamoto K (1987) Mol Gen Genet 206: 408–413
Lührs R, Lörz H (1988) Planta 175: 71–81
Maddock SE (1987) Plant Cell Report 6: 23–26
Mathias RJ, Boyd LA (1986) Plant Science 46: 217–223
Mathias RJ, Simpson ES (1986) Plant Cell, Tissue and Organ Culture 7: 31–37
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Ohira K. Ojima K. Fujiwara A (1973) Plant Cell Physiol 14: 1113–1121
Ozias-Akins P, Vasil IK (1983) Theor Appl Genet 117: 40–44
Ozias-Akins P, Lörz H (1984) Trends in Biotechnology 2: 119–123
Potrykus I, Petruska J (1983) In: Proceedings of 6th International Protoplast Symposium, Birkhauser Verlag, Basel, Boston, Stuttgart, pp 12–13
Purnhauser L, Medgyesy P, Czako M, Dix PF, Marton L (1987) Plant Cell Rep 6: 1–4
Rhodes CA, Lowe KS, Ruby KL (1988) Bio/Technology 6: 56–60
Terada R, Kyozuka J, Nishibayashi S, Shimamoto K (1987) Mol Gen Genet 210: 39–43
Toriyama K, Hinata K, Sasaki T (1986) Theor Appl Genet 73: 16–19
Vasil V, Vasil IK (1980) Theor Appl Genet 56: 97–99
Vasil IK (1987) J Plant Physiol 128: 193–218
Wei ZM (1982) Theor Appl Genet 63: 71–73
Yamada Y, Yang ZQ, Tang DT (1986) Plant Cell Rep 4: 85–88
Author information
Authors and Affiliations
Additional information
Communicated by A. R. Gould
Rights and permissions
About this article
Cite this article
Hayashi, Y., Shimamoto, K. Wheat protoplast culture: embryogenic colony formation from protoplasts. Plant Cell Reports 7, 414–417 (1988). https://doi.org/10.1007/BF00269526
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269526