Abstract
Electric pulses applied to Colt cherry protoplasts enhanced the long-term growth and plant regeneration of protoplast-derived tissues. Protoplasts isolated from long-term cultured tissues derived from electroporated protoplasts retained the ability to enter division in culture earlier and with a higher frequency of plant regeneration than untreated cell suspension protoplasts.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- GA3 :
-
gibberellic acid
- IBA:
-
4-indole-3yl-butyric acid
- MES:
-
2-N-morpholinoethane sulphonic acid
- MS:
-
Murashige and Skoog (1962)
- NAA:
-
α -naphthaleneacetic acid
- PE:
-
plating efficiency
- Z:
-
zeatin
References
Ochatt SJ, Chand PK, Rech EL, Davey MR, Power JB (1988) Plant Sci. 54: 165–169
Rech EL, Ochatt SJ, Chand PK, Power JB, Davey MR (1987) Protoplasma 141: 169–176
Rech EL, Ochatt SJ, Chand PK, Davey MR, Mulligan BJ, Power JB (1988) BioTechnology (in press)
Shillito RD, Saul MW, Paszkowski J, Muller M, Potrykus I (1985) BioTechnology 3: 1099–1103
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Communicated by I. Potrykus
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Ochatt, S.J., Rech, E.L., Davey, M.R. et al. Long-term effect of electroporation on enhancement of growth and plant regeneration of colt cherry (Prunus avium × pseudocerasus) protoplasts. Plant Cell Reports 7, 393–395 (1988). https://doi.org/10.1007/BF00269520
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DOI: https://doi.org/10.1007/BF00269520