Abstract
Microscopic observations made during preparation of protoplasts and vacuoles from red radish seedling hypocotyl (Raphanus sativus L.) show that anthocyanoplasts, the strongly pigmented bodies present in the pigmented cells of the hypodermis, begin as apparently membranous vesicles in the cytoplasm made visible by the deposition and accumulation of anthocyanins, but only rarely appear in the isolated vacuole. Isolation of protoplasts and vacuoles was also achieved from mung bean seedling hypocotyl (Vigna radiata L Wilczek), red cabbage leaf (Brassica oleracea L.) and Prunus x yedoensis Matsum callus. Anthocyanoplasts were usually in the vacuole, although sometimes in the cytoplasm, of the mung bean and cabbage, but were never seen in vacuoles of Prunus callus.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Duke SO, Vaughn KC 1982 Physiol Plant 45: 381–385
Fuleki T 1969 J Food Sci 34: 365–369
Gamborg OL 1966 Can J Biochem 44: 791–799
Heller W, Forkman G, Britsch L, Grisebach H 1985 Planta 165: 284–287
Hemleben V 1981 Z Naturforsch 36C: 925–927
Hopp W, Seitz HV 1987 Planta 170: 74–85
Hrazdina G, Zobel AM, Hoch, HC 1988 Proc Natl Acad Sci USA 84: 8966–8970
Hrazdina G, Wagner GJ 1985a Archiv Biochem Biophys 237: 88–100
Hrazdina G, Wagner GJ 1985b In: van Sumere C, Harborne JB (eds) Biochemistry of Plant Phenolics. Chapman & Hall, London, New York
Hrazdina G, Wagner GJ, Siegelman HW 1978 Phytochem 17: 53–56
Hrazdina G, Iredale H, Mattick LR 1977 Phytochem 16: 297–299
Ishikura N, Iwata M, Miyazaki S 1981 Bot Mag Tokyo 94: 197–205
Molisch H 1905 Bot Zeitung 63: 145–162
Murashige T, Skoog, F 1962 Physiol Plant 15: 473–497
Neumann D 1983 Biochem Physiol Pflanzen 178: 405–407
Nozue M, Yasuda H 1985 Plant Cell Reports 4: 252–255
Nozue M, Kawait T, Yoshitama K 1987 J Plant Physiol 129: 81–88
Parham RA, Kaustinen HM 1977 Bot Gaz 138: 465–467
Pecket RC, Small CJ 1980 Phytochem 19: 2571–2576
Small CJ, Pecket RC 1982 Planta 154: 97–99
Ulrich I, Ulrich W 1986 Z Naturforsch 41C: 1052–1056
Wagner GJ. 1985 In: Hall JL, Moore AL (eds) Isolation of membranes and organelles from plants Academic Press, London, New York pp. 83–118
Yasuda H 1987 Proc XIV Int Bot Congress Berlin 4-106-15
Yasuda H, Shinoda H 1985 Cytologia 50: 397–403
Author information
Authors and Affiliations
Additional information
Communicated by F. Constabel
Rights and permissions
About this article
Cite this article
Nozzolillo, C., Ishikura, N. An investigation of the intracellular site of anthocyanoplasts using isolated protoplasts and vacuoles. Plant Cell Reports 7, 389–392 (1988). https://doi.org/10.1007/BF00269519
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269519