Abstract
Mesophyll protoplasts obtained from leaves of shoot cultures of Rehmannia glutinosa were cultured in Murashige and Skoog (1962) liquid or liquid-over-agar medium containing 2.0 mg L−1 naphthaleneacetic acid and 0.5 mg L−1 benzylamino purine. An amino acid mixture of glutamine, arginine, glycine, and aspartic acid promoted sustained protoplast division, with an average plating efficiency of 27%. Protoplast-derived colonies formed callus which readily regenerated shoots on fransfer to Murashige and Skoog based agar medium with 2.0 mg L−1 indoleacetic acid and 1.0 mg L−1 benzylamino purine. Leaf explants also showed a marked capacity for shoot regeneration in culture.
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Abbreviations
- BAF:
-
6-benzylamino purine
- NAA:
-
α-naphthaleneacetic acid
- IAA:
-
3-indoleacetic acid
- MS,KM,UM,CPW:
-
see Methods
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Xu, XH., Davey, M.R. Shoot regeneration from mesophyll protoplasts and leaf explants of Rehmannia glutinosa . Plant Cell Reports 2, 55–57 (1983). https://doi.org/10.1007/BF00269237
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DOI: https://doi.org/10.1007/BF00269237