Abstract
We have examined the toxicity of over twenty antibiotics to protoplast-derived cells of Nicotiana plumbaginifolia. The least toxic antibiotics are the betalactams: ampicillin, carbenicillin and the cephalosporins can be used to provide broad spectrum antimicrobial activity without significant toxicity to plant cells. Similar broad spectrum activity can also be obtained by combining rifampicin and trimethoprim. Other antibiotics which may be useful are erythromycin and colistin. The aminoglycosides are not recommended.
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Abbreviations
- RH:
-
Relative Humidity
- NAA:
-
1- Naphthylacetic Acid
- BAP:
-
6- Benzylamino Purine
References
Bancroft, JB, F Motoyoshi, JW Watts and JRO Dawson (1975) In: Markham et al (eds) Modification of the information content of plant cells. North Holland Amsterdam pp145.
Caboche, M (1980) Planta 149 7–18
Dix, PJ, F Joo and P Maliga (1977) Mol. Gen. Genet 157 285–290.
Eichholtz, DA, PM Hasegwa, HA Robitaille (1982). In vitro 18 12–18.
Garrod, LP, HP Lambert, F O'Grady and PM Waterworth (1981) In: Antibiotic and Chemotherapy (5th ed) Churchill Livingstone, Edinburgh.
Kerry, DW, JMT Hamilton-Miller and W Brumfitt (1975) J. Antimicrobial Chemotherapy 1 417–427.
Laskey, RA, J. Cell. Sci. (1970) 7 653–659.
Lesley, SM, and RM Behki (1977) In Vitro 13 366–370.
Lurquin, PF, and A Kleinhofs (1982) Biochem. biophys Res. Comm 107 286–293.
Maliga, P, LT Xucan, PJ Dix and A Cséplö (1980) In: Sala F et al (eds) Plant cell cultures: results and perspectives. Elsevier/North Holland. Amsterdam p161–166.
Netein, G and M Bertrand (1959) C.R. Soc. Biol. 148 1262–1263.
Nickell, LG (1952) Proc. Soc. exptl. Biol. Med. 80 615–617.
Nicholas, P (1981) Plant Sci. Lett 22 309–316.
Phillips, R, SM Arnott and SE Kaplan (1981) Plant Sci. Lett 21 235–240.
Scowcroft, WR and PJ Larkin (1980) Aust J Plant Physiol 7 635–644.
Thurston, KC, SJ Spencer and J Arditti (1979) Am. J. Bot 66 825–835.
Ursic, D, JD Kemp and JP Helgeson (1981) Biochem biophys. Res. Comm 101 1031–1037.
Watts, JW and JM King (1973) Planta 113 271–277.
Zilkah, S and J Gressel (1977) Plant and Cell Physiol. 18 815–820.
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Pollock, K., Barfield, D.G. & Shields, R. The toxicity of antibiotics to plant cell cultures. Plant Cell Reports 2, 36–39 (1983). https://doi.org/10.1007/BF00269232
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DOI: https://doi.org/10.1007/BF00269232