Abstract
Nodal explants (0.5 to 0.8 cm long) isolated from 2-year old shrubs of guayule, Parthenium argentatum Gray, when cultured on MS medium supplemented with different concentrations of KN, BAP, 2,4-D, 2,4-D + BAP, NAA and NAA + BAP produced callus tissues and shoots simultaneously with varying frequencies. Shoots were regenerated with a high frequency (80–88%) from callus on MS medium containing NAA + BAP with or without glutamine. Addition of glutamine to these media improved considerably the number of shoots formed from a known amount of callus. Shoots could be regenerated from 200 day old callus cultures with a very high frequency but the organogenetic capacity declined thereafter. Increase in the concentration of sucrose (upto 4%) significantly enhanced the shoot forming ability of callus, but higher concentrations (6%) suppressed it. Rooting was induced only in dark when IAA, IBA and NAA were used, but 2,4-D could induce them both in light and dark. The system is suitable for the mass propagation of this important rubber yielding plant.
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Abbreviations
- MS:
-
Murashige and Skoog (1962)
- IAA:
-
Indole-3-acetic acid
- IBA:
-
Indole-3-butyric acid
- NAA:
-
α-Naphthaleneacetic acid
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- KN:
-
Kinetin
- BAP:
-
6-Benzylaminopurine
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Communicated by I. K. Vasil
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Dhar, A.C., Kavi Kishor, P.B. & Rao, A.M. In vitro propagation of guayule (Parthenium argentatum) — a rubber yielding shrub. Plant Cell Reports 8, 489–492 (1989). https://doi.org/10.1007/BF00269056
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DOI: https://doi.org/10.1007/BF00269056