Abstract
Tobacco mosaic virus-resistant tobacco was selected in vitro using callus tissues induced from axillary buds of systemically infected tobacco plants. Callus lines in which the virus was continuously multiplying were first isolated and redifferentiated into shoots. By the procedure, non-diseased, healthy shoots were successfully isolated from diseased shoots, which showed typical mosaic symptoms of the virus, and regenerated into intact plants.
These regenerated plants showed resistance to virus inoculation, and selfed progeny of virus-resistant regenerants segregated the resistance and susceptibility according to the Mendelian system.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Brettell RIS, Ingram DM (1979) Biol Rev 54:329–345.
Evans DA, Sharp WR (1983) Science 221:949–951.
Larkin PJ, Scowcroft WR (1981) Theor Appl Genet 60:197–214.
Murakishi HH, Carlson PS (1982) Plant Cell Rept 1:94–97.
Murashige T, Skoog F (1962) Physiol Plant 15:473–497.
Ogura H (1976) Jpn J Genet 51:161–174.
Shepard JF, Bindey D, Shahin EA (1980) Science 208:17–24.
Toyoda H, Oishi Y, Hirai T (1983) Ann Phytopath Soc Jpn 49:488–494.
Toyoda H, Oishi Y, Matsuda Y, Chatani K, Hirai T (1985a) Phytopath Z 114:126–133.
Toyoda H, Matsuda Y, Hirai T (1985b) Ann Phytopath Soc Jpn 51:32–38.
Toyoda H, Matsuda Y, Hirai T (1986) Plant Tissue Culture Lett 3:22–27.
Toyoda H, Shimizu K, Chatani K, Kita N, Ouchi S (1989) Plant Cell Rept (in press).
Author information
Authors and Affiliations
Additional information
Communicated by J. M. Widholm
Rights and permissions
About this article
Cite this article
Toyoda, H., Chatani, K., Matsuda, Y. et al. Multiplication of tobacco mosaic virus in tobacco callus tissues and in vitro selection for viral disease resistance. Plant Cell Reports 8, 433–436 (1989). https://doi.org/10.1007/BF00269042
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269042