Abstract
Sheaths isolated from Gloeobacter violaceus were found to be composed of a major polysaccharide moiety (glucose, galactose, rhamnose, mannose, arabinose), a protein moiety, and negatively charged components (glucuronic acids, phosphate, sulfate). Outer membrane polypeptide patterns were dominated by two major peptidoglycan-associated proteins (Mr 62,000 and 53,000). Lipopolysaccharide constituents were glucosamine, 3-hydroxy fatty acids (3-OH-14:0, anteiso-3-OH-15:0, 3-OH-16:0, 3-OH-18:0), carbohydrates, and phosphate. A1γ-type peptidoglycan and non-peptidoglycan components (mannosamine, glucose, mannose, and glucosamine) indicated the presence of a peptidoglycan-polysaccharide complex in the cell walls of Gloeobacter violaceus.
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Abbreviations
- A2pm:
-
diaminopimelic acid
- ATCC:
-
American Type Culture Collection
- CE:
-
cell envelope
- CM:
-
cytoplasmic membrane
- CW:
-
cell wall
- dOcla:
-
3-deoxy-d-manno-2-octulosonic acid
- GalN:
-
galactosamine
- GlcN:
-
glucosamine
- GlcUA:
-
glucuronic acid
- HF:
-
hydrofluoric acid
- LPS:
-
lipopolysaccharide
- ManN:
-
mannosamine
- M:
-
relative molecular mass
- MurN:
-
muramic acid
- MurN-6-P:
-
muramic acid-6-phosphate
- OMe:
-
O-methyl
- PAGE:
-
polyacrylamide gel electrophoresis
- PCC:
-
Pasteur Culture Collection
- SDS:
-
sodium dodecyl sulfate
- SH:
-
sheath
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Schneider, S., Jürgens, U.J. Cell wall and sheath constituents of the cyanobacterium Gloeobacter violaceus . Arch. Microbiol. 156, 312–318 (1991). https://doi.org/10.1007/BF00263004
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DOI: https://doi.org/10.1007/BF00263004