Summary
The sequencing and comparison of the genes encoding the glyoxylate bypass enzyme malate synthase of Aspergillus nidulans (acuE) and Neurospora crassa (acu-9) are presented. The predicted amino acid sequences of the A. nidulans and N. crassa enzymes are 538 and 542 residues respectively and the proteins are 87% homologous. In fungi, the malate synthase proteins are located in glyoxysomes and the deduced acuE and acu-9 proteins both contain a C-terminal S-K-L sequence, which has been implicated in transport into peroxisomes. The acuE coding region is interrupted by four introns and the acu-9 coding region is interrupted by one intron which occurs at the same position as the C-terminal acuE intron. The 5′ non-coding regions of the two genes were examined for short homologous sequences that may represent the binding sites for regulatory proteins. Pyrimidine-rich sequences with weak homology to the amdI9 sequence, which has been implicated in facB-mediated acetate regulation of the amdS gene, were found but their functional significance remains to be determined.
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Communicated by C.A. v. den Hondel
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Sandeman, R.A., Hynes, M.J., Fincham, J.R. et al. Molecular organisation of the malate synthase genes of Aspergillus nidulans and Neurospora crassa . Molec. Gen. Genet. 228, 445–452 (1991). https://doi.org/10.1007/BF00260638
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DOI: https://doi.org/10.1007/BF00260638