Summary
A sensitive competition radioimmunoassay using 125I-labelled p 30 interspecies antigen, antiserum specific to the interspecies antigen of the feline leukaemia virus, and aqueous tissue extracts from prostate was used to examine benign hyperplastic prostates for the presence of protein components able to compete with the interspecies viral antigens. Six of 20 prostatic nodular hyperplastic tissues were competitive in radioimmunoassay with the 125I-labelled viral antigen for binding sites on the antiviral antibodies. These findings suggest the presence of oncornavirus-like proteins in prostatic nodular hyperplasia. No correlation could be made between the presence of competing protein and histological features of acute or chronic prostatitis and squamous metaplasia.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ablin, R. J.: Immunologic studies of normal, benign, and malignant human prostatic tissue. Cancer (Philad.) 29, 1570 (1972)
Ablin, R. J., Bronson, P., Soanes, W., Witebsky, E.: Tissue- and species-specific antigens of normal human prostatic tissue. Journal of Immunology 104, 1329 (1970)
Birkmayer, G. D., Balda, B. R., Miller, F.: Oncorna-viral information in human melanoma. European Journal of Cancer 10, 419 (1974)
Flocks, R. H., Boatman, D. L., Hawtrey, C. E.: Tissue specific isoantigens in the dog prostate. Investigative Urology 10, 215 (1972)
Flocks, R. H., Urich, V. C., Patel, C. A., Opitz, J. M.: Studies on the antigenic properties of prostatic tissue. Journal of Urology 34, 134 (1960)
Green, R. W., Bolognesi, D. P.: Isolation of proteins by gel filtration in 6 M Guanidinium chloride. Application to RNA tumor viruses. Analytical Biochemistry 57, 108 (1974)
Greenwood, F. C., Hunter, W. M.: The preparation of 131I-labelled human growth hormone of high specific radioactivity. Biochemical Journal 89, 114 (1963)
Hyin, K. V., Irlin, I. S., Bykovsky, A. F., Spure, Z. Z., Miller, G. G., Abenova, U. A., Zhdanov, V. M.: Immunologic study of an oncornavirus isolated from a human cancer cell line. Cancer (Philad.) 34, 532 (1974)
Mickey, D. D., Seal, E., Jr., Paulson, D. F.: Detection of oncornavirus antigenic activity in human urothelial tissues. Journal of Urology 113, 658 (1975)
Sherr, C. J., Todaro, G. J.: Type C viral Antigens in man. I. Antigens related to endogenous primate virus in human tumors. Proceedings of the National Academy of Science of the United States of America 71, 4703 (1974)
Strand, M., August, J. T.: Structural proteins of oncogenic RNA viruses: Interspec II, a new interspecies antigen. Journal of Biological Chemistry 248, 5627 (1973)
Strand, M., August, J. T.: Type-C RNA virus gene expression in human tissue. Journal of Virology 14, 1584 (1974)
Author information
Authors and Affiliations
Additional information
Supported by N. I. H. Grant Nos. 5 R26 CA15292 and 5 R26 CA15417
Rights and permissions
About this article
Cite this article
Seal, E., Mickey, D.D. & Paulson, D.F. Oncornavirus-like protein expression in human prostatic tissue. Urol. Res. 4, 23–27 (1976). https://doi.org/10.1007/BF00256132
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00256132