Summary
The effects of intravenous (iv) inoculation of rat prostate R3327-MatLyLu tumor cells in syngenic Copenhagen rats have been investigated both qualitatively and quantitatively in order to establish an in vivo clonogenic cell assay. Intravenous injection of tumor cells resulted in formation of tumor nodules only in the lungs. A linear relationship existed between the number of tumor cells injected and the number of measured lung nodules. The distribution patterns of iv injected radiolabelled tumor cells and of non-labelled tumor cells injected in both arterial and venous blood vessels confirmed the major role of the lungs as recipient organ for injected tumor cells. Although a similar distribution pattern was observed after iv injection with radiolabelled fibroblasts, no lung nodules were observed even one year after injection. The sensitivity for measuring drug treatment effects by this in vivo clonogenic cell assay has been compared with that of the in vitro clonogenic cell assay. Information obtained using both in vitro and in vivo clonogenic cell assay will provide a better understanding of efficacy of treatment modalities.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Geldof AA, Rao BR, Voogt de HJ (1986) Direct effects of chemotherapeutic agents on rat prostate tumor clonogenic cells. Anticancer Res 6:837–840
Hanna N (1982) Metastasis and allogeneic tumors in nude mice. In: Liotta L, Hart IR (eds) Tumor invasion and metastasis. Nijhoff, The Hague Boston London, pp 43–56
Hart IR (1982) The role of animal models in the study of experimental metastasis. In: Liotta LA, Hart IR (eds) Tumor invasion and metastasis. Nijhoff, The Hague Boston London, pp 1–14
Isaacs JT, Yu GW, Coffey DS (1981) The characterization of a newly identified, highly metastatic variety of Dunning R3327 rat prostatic adenocarcinoma system: The MatLyLu tumor. Invest Urol 19:20–23
Lucké B, Breedis C, Woo ZP, Berwick L, Nowell P (1952) Differential growth of metastatic tumors in liver and lung. Experiments with rabbit V2 carcinoma. Cancer Res 12:734–738
Mellgren J (1976) Quantitation of metastases in experimental animals. In: Weiss L (ed) Fundamental aspects of metastasis. North-Holland, Amsterdam Oxford, pp 243–252
Rao BR, Nakeff A, Eaton C, Heston WDW (1978) Establishment and characterization of an in vitro clonogenic cell assay for the R-3327-AT Copenhagen rat prostatic tumor. Cancer Res 38:4431–4439
Salmon SE, Alberts DS, Meyskens FL, Durie BGM, Jones SE, Soehnlen B, Young L, Chen HSG, Moon TE (1980) Clinical correlations of in vitro drug sensitivity. In: Salmon SE (ed) Cloning of human tumor stem cells. Alan R Liss, New York, pp 223–245
Steele GG (1977) Growth and survival of tumor stem cells. In: Steele GG (ed) Growth kinetics of tumors. Clarendon Press, Oxford, pp 217–262
Till JE, McCulloch EA (1961) A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res 14:213–222
Warenius HM, Bleehen NM (1982) In vivo-in vitro clonogenic assays in a human tumour xenograft with a high plating efficiency. Br J Cancer 46:45–50
Weiss L (1984) Overview of the metastatic cascade. In: Honn KV, Sloane BF (eds) Hemostatic mechanisms and metastasis. Nijhoff, Boston, pp 15–39
Wexler H (1966) Accurate identification of experimental pulmonary metastases. J Natl Cancer Inst 36:641–645
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Geldof, A.A., Rao, B.R. & de Voogt, H.J. Development of an in vivo clonogenic cell assay for rat prostate metastatic tumor—R3327-MatLyLu. Urol. Res. 15, 139–144 (1987). https://doi.org/10.1007/BF00254425
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00254425