Summary
Growth limiting factors influencing the high density cultivation of insect cells were studied. A stationary phase in Grace's medium was found to be due to the deprivation of the active form of FBS components. Various compounds were added in the early stationary phase to observe the recovery of cell growth. With the addition of yeastolate, final cell densities were 4-fold and 3.5-fold higher in monolayer and suspension cultures, respectively. Pluronic F-68 increases the specific growth rate and the growth yield of the cell as well as protects the cell from the shear damage.
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Agathos, S.N., Jeong, Y-H., and Venkat, K. (1990). In: Annals of the New York Academy of Sciences Biochemical Engineering VI, W.E. Goldstein, D. DiBiasio, and H. Pedersen, eds. vol 589, pp 372–398, New York: The New York Academy of Sciences.
Bentley, P.K., Gates, R.M.C., Lowe, K.C., de Pomerai, D.I., and Walker, J.A.L. (1989). Biotechnol Lett. 11, 111–114.
Glacken, M.W., Adema, E., and Sinsky, A.J. (1989). Biotechnol. Bioeng. 33, 440–450.
Hink, W.F. (1970). Nature, 226, 466–467.
King, G.A., Daugulis, A.J., Faulkner, P., and Goosen, M.F.A. (1988). Biotechnol Lett. 10, 683–688.
Kloppinger, J., Fertig, G., Fraune, E., and Miltenburger, H.G. (1990). In: Production of biologicals from animal cells in culture, W.E. Spier, J.B. Griffiths, and B. Meigner, eds. pp 470–475, Oxford: Butterworth-Heinemann Press.
Lindsay, D.A. and Betenbaugh, M.J. (1992). Biotechnol. Bioeng. 39, 614–618.
Maiorella, B., Inlow, D., Shauger, A., and Harano, D. (1988). Bio/Technology 6. 1406–1410.
Murhammer, D.W. and Goochee, C.F. (1988). Bio/Technology 6. 1411–1418.
Shuler, M.L. (1992). In: Biochemical Engineering for 2001, S. Furusaki, I. Endo, and R. Matsuno, eds. pp 309–314, Tokyo: Springer-Verlag.
Summers, M.D. and Smith, G.E. (1987). A mannual method for baculovirus vectors and insect cell culture procedures, Texas Agricultural Experiment Station Bulletin No. 1555, Texas A&M University, College Station, Texas.
Wu, J., King, G., Daugulis, A.J., Faulkner, P., Bone, D.H., and Goosen, M.F.A. (1989). Appl. Microbiol. Biotechnol. 32, 249–255.
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Lee, S.H., Park, T.H. Growth limiting factors influencing high density culture of insect cells in Grace's medium. Biotechnol Lett 16, 327–332 (1994). https://doi.org/10.1007/BF00245045
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DOI: https://doi.org/10.1007/BF00245045