Summary
A full-length cDNA clone encoding tomato (Lycopersicon esculentum Mill.) 5-aminolevulinic acid dehydratase (ALAD) was isolated and characterized. The primary structure predicts a 430-amino acid precursor which comprises a 41.7 kDa, 388-amino acid mature protein and a 47-amino acid transit sequence. The tomato primary sequence shows extensive homology to those of pea and spinach. Southern analysis indicated that 1 to 2 copies of the ALAD gene are present in the tomato genome. Northern blot analysis shows differential expression in various tomato organs, and constitutive developmental expression in tomato fruits.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- ALA:
-
5-aminolevulinic acid
- ALAD:
-
5-aminolevulinic acid dehydratase (EC 4.2.1.24)
References
Beale SI, Einstein JD (1990) In: Dailey HA (ed) Biosynthesis of Heme and Chlorophylls, McGraw-Hill, Inc., USA, pp 287–391
Boese QF, Spano AJ, Li J, Timko MP (1991) J. Biol. Chem. 266:17060–17066
Dix KP, Rawson JRY (1983) Curr. Genet. 7:265–272
Echelard Y, Dymetryszyn J, Drolet M, Sasarman A (1988) Mol. Gen. Genet. 214:503–508
Gibbs PNB, Jordan PM (1986) Biochem. J. 236:447–451
Huault C, Aoues A, Colin P (1987) Plant Physiol. Biochem. 25:723–728
Kasemir H, Masoner M (1975) Planta 126:119–126
Kotzabasis K, Breu V, Dörnemann D (1989) Biochim. Biophys. Acta 977:309–314
Leeper FJ (1991) In: Scheer H (ed) Chlorophylls, CRC Press, Boca Raton, FL, pp 407–431
Liedgens W, Grützmann R, Schneider HAW (1980) Z. Naturforsch. 35c:958–962
Liedgens W, Lütz C, Schneider HAW (1983) Eur. J. Biochem. 135:75–79
Perlman D, Halvorson HO (1983) J. Mol. Biol. 167:391–409
Phua SH, Srinivasa BR, Subramanian AR (1989) J. Biol. Chem. 264:1968–1971
Richards WR (1993) In: Sundqvist C, Ryberg M (eds) Pigment-Protein Complexes in Plastids, Academic Press, Inc., San Diego, CA, pp 91–178
Rogers SO, Bendich AJ (1985) Plant Mol. Biol. 5:69–76
Sanger F, Nicklen S, Coulson AR (1977) Proc. Natl. Acad. Sci. USA 74:5463–5467
Schaumburg A, Schneider-Poetsch HAW, Eckerskorn C (1992) Z. Naturforsch. 47c:77–84
Schmidt GW, Mishkind ML (1986) Ann. Rev. Biochem. 55:879–912
Shibata H, Ochiai H (1976) Plant Cell Physiol. 17:281–288
Shioi Y, Doi M (1988) Plant Cell Physiol. 29:843–848
Smith AG (1988) Biochem. J. 249:423–428
Spano AJ, He Z, Timko MP (1992) Mol. Gen. Genet. 236:86–95
Spano AJ, Timko MP (1991) Biochim. Biophys. Acta 1076:29–36
Tchuinmogne SJ, Huault C, Aoues A, Balangé AP (1989) Plant Physiol. 90:1293–1297
Thomas P (1980) Proc. Natl. Acad. Sci. USA 84:744–748
van Heyningen S, Shemin D (1971) Biochem. 10:4676–4682
Wetmur JG, Bishop DF, Cantelmo C, Desnick RJ (1986) Proc. Natl. Acad. Sci. USA 83:7703–7707
Author information
Authors and Affiliations
Additional information
Communicated by C.F. Quiros
Rights and permissions
About this article
Cite this article
Polking, G.F., Hannapel, D.J. & Gladon, R.J. Characterization of a cDNA encoding 5-aminolevulinic acid dehydratase in tomato (Lycopersicon esculentum Mill.). Plant Cell Reports 14, 366–369 (1995). https://doi.org/10.1007/BF00238598
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00238598