Summary
Variations in the ploidy level of 69 transgenic potato (Solanum tuberosum L.) plants regenerated from the tuber discs of 17 diploid lines were studied: 24 plants (35%) were diploid, the other 45 plants (65%) were tetraploid. Seventy-eight control regenerants obtained without Agrobacterium inoculation showed a relatively low tendency to tetraploidization (35%). The results obtained suggested that chromosome doubling occurred frequently in diploid potato lines during the tissue culture process for regeneration. Putative somaclonal changes in in vitro-formed tuber proteins were detected in three out of six transformants by electrophoretic analysis.
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References
Aida R, Takaiwa F, Imai T, Ishige T (1991) Japan J Breed 41: 511–515
Dale PJ, McPartlan HC (1992) Theor Appl Genet 84: 585–591
De Block M (1988) Theor Appl Genet 76: 767–774
Debnath SC, Schuchmann R, Wenzel G (1986) Acta Bot Neerl 35: 233–241
Debnath SC, Wenzel G (1987) Potato Research 30: 371–381
Hänisch ten Cate ChH, Ennik E, Roest S, Sree Ramulu K, Dijkhuis P, Groot Bde (1988) Theor Appl Genet 75: 452–459
Imai T, Irikura Y, Ishige T (1992) Plant Cell Tissue Organ Culture 29: 115–124
Ishige T, Ohshima M, Ohashi Y (1991) Plant Science 73: 167–174
Leij FR van der, Visser RGF, Oosterhaven K, Kop DAM van der, Jacobsen E, Feenstra WJ (1991) Theor Appl Genet 82: 289–295
Mattheij WM, Eijlander R, Koning JRA de, Louwes KM (1992) Theor Appl Genet 83: 459–466
Sheerman S, Bevan MW (1988) Plant Cell Reports 7: 13–16
Stiekema WJ, Heidekamp F, Louwerse JD, Verhoeven HA, Dijkhuis P (1988) Plant Cell Reports 7: 47–50
Visser RGF, Hesseling-Meinders A, Jacobsen E, Nijdam H, Witholt B (1989a) Theor Appl Genet 78: 705–714
Visser RGF, Jacobsen E, Hesseling-Meinders A, Schans MJ, Witholt B, Feenstra WJ (1989b) Plant Mol Biol 12: 329–337
Vries-Uijtewaal E de, Gillssen LJW, Flipse E, Sree Ramulu K, Groot B de (1988) Plant Science 58: 193–202
Vries-Uijtewaal E de, Gilissen LJW, Flipse E, Sree Ramulu K, Stiekema WJ, Groot B de (1989) Theor Appl Genet 78: 185–193
Waara S, Wallin A, Ottosson A, Eriksson T (1991) Plant Cell Tissue Organ Culture 27: 257–265
Wenzel G, Schieder O, Przewozny T, Sopory SK, Melchers G (1979) Theor Appl Genet 55: 49–55
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Communicated by M. Tabata
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Imai, T., Aida, R. & Ishige, T. High frequency of tetraploidy in Agrobacterium-mediated transformants regenerated from tuber discs of diploid potato lines. Plant Cell Reports 12, 299–302 (1993). https://doi.org/10.1007/BF00237422
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DOI: https://doi.org/10.1007/BF00237422