Summary
Genotypes of Lolium perenne L. with different androgenetic responses were used to test effects of induction medium composition. The media tested were potato II (pII), 190-2, and modified Linsmaier and Skoog media, LS-1, LS-2, and LS-3. The effect of different gelling agents, activated charcoal in a ‘double layer’ design, and casein hydrolysate were also studied. From 36,696 anthers, 25,906 embryo-like structures, 1,959 albino and 173 green plants were generated. Significant differences were found between media, genotypes and medium-genotype interactions studied. All three media commonly used, pII, 190-2, and LS-3, were equivalent in production of green plants. Cold pretreatment of the anthers (4°C) significantly increased the number of embryo-like structures, the number and proportion of albino plants produced, but not the production of green plants.
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Abbreviations
- ELS:
-
embryo-like structures
- ALB:
-
albino plants
- ANT:
-
anthers
- GRP:
-
green plants
- DH:
-
doubled haploid plants
- AC:
-
activated charcoal
- CH:
-
casein hydrolysate
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- LS:
-
Linsmaier and Skoog (1965) basal medium
- MS:
-
Murashige and Skoog (1962)
- PL:
-
plants
- pII:
-
potato II induction medium
- DL:
-
double layer
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Opsahl-Ferstad, HG., Bjørnstad, Å. & Rognli, O.A. Influence of medium and cold pretreatment on androgenetic response in Lolium perenne L.. Plant Cell Reports 13, 594–600 (1994). https://doi.org/10.1007/BF00234518
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DOI: https://doi.org/10.1007/BF00234518