Abstract
Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10−7, 10−6 and 10−5M). Adventitious shoots differentiated from callus developed from the cut ends of 12.5% of the hypocotyl segments cultured on medium supplemented with 10−6M BAP in combination with 10−6M 2,4-D or 10−6M NAA. Profuse rooting occurred from the hypocotyl explants on medium supplemented with 10−6M BAP + 10−6M NAA. Incorporation of casein hydrolysate in B5 medium along with 10−6M BAP + 10−7M 2,4-D enhanced the frequency of cultures with adventitious shoots upto 68.0%. Glutamine, glutamic acid or proline could partially substitute for the effect of casein hydrolysate. Amongst the hypocotyls from 3–14 d old seedlings, the best caulogenesis was obtained with hypocotyls from 7 d old seedlings both in presence or absence of casein hydrolysate. Best rooting of shoots was achieved on half-strength B5 medium supplemented with 10−6M IBA. After hardening, plantlets were successfully transferred to the soil.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2, 4-dichlorophenoxyacetic acid
- IBA:
-
indole-3-butyric acid
- NAA:
-
1-naphthaleneacetic acid
- CH:
-
casein hydrolysate
- Arg:
-
L-arginine
- Glu:
-
L-glutamic acid
- Gln:
-
L-glutamine
- Leu:
-
L-leucine
- Lys:
-
L-lysine
- Pro:
-
L-proline
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Rajasubramaniam, S., Pardha Saradhi, P. Organic nitrogen stimulates caulogenesis from hypocotyl callus of Phyllanthus fraternus . Plant Cell Reports 13, 619–622 (1994). https://doi.org/10.1007/BF00232932
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DOI: https://doi.org/10.1007/BF00232932