Summary
Backcross and F2 progenies were produced between two bean genotypes, ‘XR-235’ and ‘Calima,’ which differ in seed weight by a factor of two. The small-seeded ‘XR-235’ was used as the pistillate and recurrent parent. These genotypes showed polymorphisms at nine isozyme loci and at the phaseolin locus. Seed size parameters (weight, length, width, and thickness) were determined for each BC1 and F2 individual, i.e., for seeds harvested from ‘XR-235’ after pollination with F1 and from the F1 after selfing, respectively. A combination of starch gel electrophoresis and enzyme activity staining was used to determine the genotype of each BC1 and F2 individual at the segregating loci. SDS-PAGE and Coomassie blue staining were used to determine geno-type at the phaseolin locus. Tests for independent assortment using two-way contingency and maximum likelihood tables revealed three linkage pairs: Aco-1 — 20 cM — Dia-1; Adh-1 — 2 cM — Got-2; and Est-2 — 11 cM — Pha. Statistical comparisons were made between the means of genotype classes at each segregating locus for all seed size parameters. The results from two independently obtained BC1s and the F2 consistently indicated that the Adh-1-Got-2 segment was linked to a locus that affected seed size and overcame maternal control over seed size. This locus has been designated Ssz-1. This gene exhibited additive gene action and accounted for 30–50% of the seed size difference between the parents.
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References
Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–278
Al-Mukhtar FA, Coyne DP (1981) Inheritance and association of flower, ovule, seed, pod, and maturity characters in dry edible beans (Phaseolus vulgaris L.). J Am Soc Hortic Sci 106:713–719
Bassett MJ (1982) A dwarfing gene that reduces seed weight and pod length in common bean. J Am Soc Hortic Sci 197:1058–1061
Conti L (1982) Bean germ plasm evaluation from the collection at Minoprio (Como, Italy) in view of a breeding program for the improvement of the proteic content of the seed. Genet Agrar 35:375–392
Conti L (1985) Conclusive results of a selection program for obtaining a dwarf bean (P. vulgaris) resistant to some viruses and characterized by agronomical qualities. Genet Agrar 39:51–63
Coyne DP (1968) Correlation, heritability, and selection of yield components in field beans. Phaseolus vulgaris L. Proc Am Soc Hortic Sci 93:388–396
Freytag GF, Bassett MJ, Zapata M (1982) Registration of XR-235–1–1 bean germ plasm. Crop Sci 22:1268–1269
Gepts P (1988) Phaseolin as an evolutionary marker. In: Gepts P (ed) Genetic resources of Phaseolus beans. Kluwer Academic, Dordrecht, pp 215–241
Hamblin J, Morton JR (1977) Genetic interpretations of the effects of bulk breeding on four populations of beans (Phaseolus vulgaris L.). Euphytica 26:75–83
Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T 4. Nature 227:680–685
Motto M, Soressi GP, Salamini F (1978) Seed size inheritance in a cross between wild and cultivated common beans (Phaseolus vulgaris L.). Genetica 49:31–36
Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726
Sax K (1923) The association of size differences with seed coat pattern and pigmentation in Phaseolus vulgaris. Genetics 8:552–560
Shapiro SS, Wilk MB (1965) An analysis of variance test for normality. Biometrika 52:591–611
Sprecher SL, Vallejos CE (1989) System/tissue/enzyme combination for starch gel electrophoresis of bean. Annu Rep Bean Improv Coop 32:32–33
Stephens MA (1974) EDF statistics for goodness of fit and some comparisons. J Am Stat Assoc 69:730–737
Tanksley SD (1984) Aconitase isozymes — new gene markers for tomato. Rep Tomato Genet Coop 34:16–18
Tanksley SD, Medina-Filho H, Rick CM (1982) Use of naturally occurring enzyme variation to detect and map genes controlling quantitative traits in an interspecific backcross of tomato. Heredity 49:11–25
Vallejos CE (1983) Enzyme activity staining. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, vol 1A. Elsevier, Amsterdam, pp 469–516
Weeden NF (1984) Distingushing among white-seeding bean cultivars by means of allozyme genotypes. Euphytica 33:199–208
Weeden NF (1986) Genetic confirmation that the variation in the zymograms of 3 enzyme systems is produced by allelic polymorphism. Annu Rep Bean Improv Coop 29:117–118
Yarnell SH (1965) Cytogenetic of the vegetable crops. IV. Legumes. Bot Rev 31: 247–330
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Communicated by F. Salamini
Florida Agricultural Experiment Station, Journal Series No. R00696
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Vallejos, C.E., Chase, C.D. Linkage between isozyme markers and a locus affecting seed size in Phaseolus vulgaris L.. Theoret. Appl. Genetics 81, 413–419 (1991). https://doi.org/10.1007/BF00228685
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DOI: https://doi.org/10.1007/BF00228685