Summary
The powdery mildew disease resistance gene Ml(La) was found to belong to a locus on barely chromosome 2. We suggest that this locus be designated MlLa. Linkage analysis was carried out on 72 chromosome-doubled, spring-type progeny lines from a cross between the winter var ‘Vogelsanger Gold’ and the spring var ‘Alf’. A map of chromosome 2 spanning 119cM and flanked by two peroxidase gene loci was constructed. In addition to the Laevigatum resistance locus the map includes nine RFLP markers, the two peroxidase gene loci and the six-row locus in barley.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Andersen S, Andersen K (1973) Linkage between marker genes on barley chromosome 2 and gene for resistance to Heterodera avenae. Hereditas 73:271–276
Bryngelson T, Collinge DB (1991) Biochemical and molecular analysis of the response of barley to infection by powdery mildew. In: Shewry P (ed) Barley: genetics, molecular biology and biotechnology. CAB International, Wallingford, England, pp 452–473
Doll H, Haahr V, Søgaard B (1989) Relationship between vernalization requirement and winter hardiness in doubled haploids of barley. Euphytica 42:209–213
Dros J (1957) The creation and maintenance of two spring barley varieties. Euphytica 6:45–48.
Feinberg A, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Giese H, Christiansen SK, Jensen HP (1990) Extrachromosomal plasmid-like DNA in the obligate parasitic fungus Erysiphe graminis f.sp. hordei. Theor Appl Genet 79:56–64
Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrmann RG (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256
Haahr V, Skou JP, Jensen HP (1989) Inheritance of resistance to barley leaf stripe (Drechslera graminea). Vortr Pflanzenzuecht 15, abstr no 3–15, p 2
Hart GE, Gale MD (1988) Guidelines for nomenclature of biochemical/molecular markers. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Agricultural and Food Research Council, Institute of Plant Science Research, Cambridge, England, pp 1215–1221
Islam AKMR, Shepherd KW, Sparrow DHB (1981) Isolation and characterization of euplasmic wheat-barley chromosome addition lines. Heredity 46:161–174
Jensen HP, Jensen J, Jørgensen JH (1991) On the genetics of ‘Laevigatum’ powdery mildew resistance in barley. In: Munck L (ed) Barley genet VI, vol 1. Munksgård Int Publ, Copenhagen, pp 593–595
Jensen HP, Christensen E, Jørgensen JH (1992) Powdery mildew resistance genes in 127 Northwest European barley varieties. Plant Breed 108:210–228
Jensen J (1987) Linkage map of barley chromosome 4. In: Yasuda S, Konishi T (eds) Barley genet V. Sanyo Press, Nakasangi Okoyama, pp 189–199
Jørgensen JH (1983) Durability of barley powdery mildew resistance genes in Denmark 1963–1980. In: Lamberti F, Waller JM, Van der Graff NA (eds) Durable resistance in crops. Plenum Publ, New York, pp 397–399
Maniatis T, Fritsch EF, Sambrook I (1982) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory Press. Cold Spring Harbor, N.Y.
Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78
Rasmussen SK, Johansson A, Rasmussen HN, Theilade B (1991) Molecular analysis and cloning of barley peroxidase genes. In: Lobarzewski J, Greppin H, Gaspar T, Penel C (eds) Biochemical, molecular and physiological aspects of plant peroxidases. University of Geneva, Geneva, pp 21–29
Röbbelen G, Abdel-Hafez AG, Reinhold M, Kwon HJ, Neuhaus-Steinmetz JP, Heun M (1983) Studies on induced partially resistant mutants of barley against powdery mildew. In: Induced mutation for disease resistance in crop plants. II. Int Atomic Energy Agency. Vienna, pp 125–138
Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of α-amylase sequences in wheat and its relatives. Theor Appl Genet 75:286–290
Shin JS, Chao S, Corpuz L, Blake T (1990) A partial map of the barley genome incorporating restriction fragment length polymorphism, polymerase chain reaction, isozyme, and morphological marker loci. Genome 33:803–810
Swanston JS (1987) The consequences, for malting quality, of Hordeum laevigatum as a source of mildew resistance in barley breeding. Ann Appl Biol 110:351–355
Torp J, Jensen HP, Jørgensen JH (1978) Powdery mildew resistance genes in 106 North-west European spring barley varieties. Kgl Vet og Landbohøjsk Årsskr 1978:75–102
Author information
Authors and Affiliations
Additional information
Communicated by P.M.A. Tigerstedt
Rights and permissions
About this article
Cite this article
Giese, H., Holm-Jensen, A.G., Jensen, H.P. et al. Localization of the Laevigatum powdery mildew resistance gene to barley chromosome 2 by the use of RFLP markers. Theoret. Appl. Genetics 85, 897–900 (1993). https://doi.org/10.1007/BF00225035
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225035