Summary
Pit cells — a new type of cell first described here and so named because they contain highly characteristic granules — are situated in the wall of rat liver sinusoids, and have hyaloplasmic pseudopodia intermingling with the microvilli of the parenchymal cells. The characteristic granules are mainly situated at one side of the nucleus, the other side showing organelle-free hyaloplasm. Pit cells are also found in portal tracts and in granuloma-like cellular aggregates. They also occur in rat peripheral blood, although there are morphological differences between cells in these two sites. Pit cells can be regarded as regular inhabitants of the sinusoidal wall, and therefore belong to the series of sinusoidal cells, i.e., the endothelial (Wisse, 1972), Kupffer (Widmann et al., 1972; Wisse and Daems, 1970; Wisse, 1974a,b), and fat-storing cells (Ito, 1973). Pit cells do not phagocytose and do not react to a great number of experimental conditions, to which endothelial and Kupffer cells do react (Wisse, 1972, 1974b). Mitosis has been observed in a pit cell.
The function of pit cells remains obscure, but an endocrine function is suggested by the morphology of their highly characteristic granules.
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Forssmann, W.G., Orci, L., Pictet, R., Renold, A.E., Rouiller, C.: The endocrine cells in the epithelium of the gastro-intestinal mucosa of the rat. J. Cell Biol. 40, 692–715 (1969)
Hoffer, A.P., Hamilton, D.W., Fawcett, D.W.: The ultrastructure of the principal cells and intraepithelial leucocytes in the initial segment of the rat epididymis. Anat. Rec. 175, 169–202 (1973)
Ito, T.: Recent advances in the study on the fine structure of the hepatic sinusoidal wall; a review. Gunma Rep. Med. Sci. 6, 119–163 (1973)
Martinez, I., Racotta, R., Russek, M.: Hepatic chromaffin cells. Life Sciences 15, 267–271 (1974)
Mills, D.M., Zucker-Franklin, D.: Electron microscopic study of isolated Kupffer cells. Amer. J. Path. 54, 147–166 (1969)
Rambourg, A.: An improved silver methenamine technique for the detection of periodic acid — reactive complex carbohydrates with the EM. J. Histochem. Cytochem. 15, 409–412 (1967)
Widmann, J.J., Cotran, R.S., Fahimi, H.D.: Mononuclear phagocytes (Kupffer cells) and endothelial cells. Identification of two functional cell types in rat liver sinusoids by endogenous peroxidase activity. J. Cell Biol. 52, 159–170 (1972)
Wisse, E.: An electron microscopic study of the fenestrated endothelial lining of rat liver sinusoids. J. Ultrastruct. Res. 31, 125–150 (1970)
Wisse, E.: An ultrastructural characterization of the endothelial cell in rat liver sinusoid under normal and various experimental conditions, as a contribution to the distinction between endothelial and Kupffer cells. J. Ultrastruct. Res. 38, 528–562 (1972)
Wisse, E.: Observations on the fine structure and peroxidase cytochemistry of normal rat liver Kupffer cells. J. Ultrastruct. Res. 46, 393–426 (1974a)
Wisse, E.: Kupffer cell reactions in rat liver under various conditions as observed in the electron microscope. J. Ultrastruct. Res. 46, 499–520 (1974b)
Wisse, E., Daems, W.Th.: Fine structural study of the sinusoidal lining cells of rat liver. In: Mononuclear phagocytes (R. van Furth, ed.), p. 200–210. Oxford: Blackwell 1970
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We thank Mrs. M. de Gruil, Mr. G.Th. Scholte, Mr. L.D.C. Verschragen and J.J. Beentjes for their assistance
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Wisse, E., van't Noordende, J.M., van der Meulen, J. et al. The pit cell: Description of a new type of cell occurring in rat liver sinusoids and peripheral blood. Cell Tissue Res. 173, 423–435 (1976). https://doi.org/10.1007/BF00224305
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DOI: https://doi.org/10.1007/BF00224305