Abstract
Near-isogenic lines (NILs) are a valuable resource for detecting linkages between qualitative trait loci and molecular markers. Molecular marker studies are expensive and methods that require genotyping fewer individuals, such as the NIL-analysis method, are desirable. We present a theory for using sets of NILs to detect linkages between molecular markers and introgressed loci. The probability that a marker a specific distance from the introgressed gene will have a donor parent allele in a near-isogenic line is a function of the distance between the marker and the gene, and the number of back-crosses and/or selfs used in deriving the NIL. The binomial probability formula is used to calculate the probability of having a donor parent allele at a given marker when sets of NILs are used. The formulae given allow calculation of the probability that a marker is linked to the introgressed gene, as well as the probability that a gene will be successfully detected when using given numbers of NILs, backcrosses, and molecular markers.
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Communicated by F. Salamini
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Kaeppler, S.M., Phillips, R.L. & Kim, T.S. Use of near-isogenic lines derived by backcrossing or selfing to map qualitative traits. Theoret. Appl. Genetics 87, 233–237 (1993). https://doi.org/10.1007/BF00223770
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DOI: https://doi.org/10.1007/BF00223770